Ribeiro, Marisa O.;
Godoy, Daniela T.;
Fontana-Maurell, Marcela;
Costa, Elaine M.;
Andrade, Elisabete F.;
Rocha, Daniele R.;
Ferreira, Antônio G.P.;
Brindeiro, Rodrigo;
Tanuri, Amilcar;
Alvarez, Patrícia.
ABSTRACT In response to the Zika epidemics in Brazil, the ZDC molecular assay (Bio-Manguinhos) was developed and registered at the Brazilian Regulatory Agency of Health Surveillance - ANVISA. The circulation of Zika (ZIKV) Dengue (DENV) and Chikungunya (CHIKV) viruses and their clinical similarities are ...
Objective: To describe the entry of Dengue virus (DENV) serotypes in Brazil and its federative units. Methods: A systematic review of studies published between 1980 and 2018 in databases and in the gray literature was performed using descriptors related to the years of entry of the DENV serotypes. Addit...
Abstract Many regions of the world where dengue epidemics are seasonal are also facing the COVID-19 pandemic. This is a medical concern because both diseases are difficult to distinguish since they have similar clinical symptoms and laboratory findings, and because they have different clinical management...
Coinfección,
Infecciones por Coronavirus,
Virus del Dengue,
Dengue,
Pandemias,
Neumonía Viral,
Betacoronavirus,
COVID-19,
Técnicas de Laboratorio Clínico,
Dengue/complicaciones,
Dengue/diagnóstico,
Virus del Dengue/genética,
SARS-CoV-2
ABSTRACT Dengue has been a significant public health problem in Colombia since the simultaneous circulation of the four dengue virus serotypes. The replicative fitness of dengue is a biological feature important for virus evolution and contributes to elucidating the behavior of virus populations and vira...
Línea Celular,
Supervivencia Celular,
Células Cultivadas,
Colombia,
Virus del Dengue/genética,
Virus del Dengue/patogenicidad,
Citometría de Flujo,
Formazáns,
Hígado/citología,
ARN Viral/genética,
Valores de Referencia,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Serogrupo,
Sales de Tetrazolio,
Factores de Tiempo,
Ensayo de Placa Viral,
Replicación Viral/genética
Iani, Felipe Campos de Melo;
Caetano, Ana Carolina Barbosa;
Cocovich, Jéssica Caroline Wenceslau;
Amâncio, Frederico Figueiredo;
Pereira, Maira Alves;
Adelino, Talita Émile Ribeiro;
Caldas, Sérgio;
Silva, Marcos Vinícius Ferreira;
Pereira, Glauco de Carvalho;
Duarte, Myrian Morato.
BACKGROUND The heat-labile nature of Dengue virus (DENV) in serum samples must be considered when applying routine diagnostic tests to avoid issues that could impact the accuracy of test results with direct implications for case management and disease reporting. OBJECTIVES To check if pre-analytical v...
Anticuerpos Antivirales/sangre,
Antígenos Virales/sangre,
Antígenos Virales/inmunología,
Dengue/sangre,
Dengue/diagnóstico,
Dengue/virología,
Virus del Dengue/genética,
Virus del Dengue/inmunología,
Virus del Dengue/aislamiento & purificación,
Ensayo de Inmunoadsorción Enzimática/métodos,
Pruebas Inmunológicas/métodos,
Valor Predictivo de las Pruebas,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos,
Sensibilidad y Especificidad,
Proteínas no Estructurales Virales/genética,
Proteínas no Estructurales Virales/inmunología
de Jesus, Jaqueline Goes;
Dutra, Karina Rocha;
Sales, Flavia Cristina da Silva;
Claro, Ingra Morales;
Terzian, Ana Carolina;
Candido, Darlan da Silva;
Hill, Sarah C;
Thézé, Julien;
Torres, Celeste;
DAgostini, Tatiana Lang;
Felix, Alvina Clara;
Reis, Andreia F Negri;
Alcantara, Luiz Carlos Junior;
de Abreu, André L;
Croda, Júlio HR;
de Oliveira, Wanderson K;
de Filipis, Ana Maria Bispo;
Camis, Maria do Carmo Rodrigues dos Santos;
Romano, Camila Malta;
Loman, Nick J;
Pybus, Oliver G;
Sabino, Ester Cerdeira;
Nogueira, Mauricio L;
Faria, Nuno Rodrigues.
BACKGROUND Despite efforts to mitigate the impact of dengue virus (DENV) epidemics, the virus remains a public health problem in tropical and subtropical regions around the world. Most DENV cases in the Americas between January and July 2019 were reported in Brazil. São Paulo State in the southeast of ...
Abstract INTRODUCTION: DENV-2 is the cause of most dengue epidemics worldwide and is associated with severe cases. METHODS: We investigated arboviruses in 164 serum samples collected from patients presenting with clinical symptoms of dengue fever and 152 mosquito pools. RESULTS: We detected the Asi...
Abstract INTRODUCTION: In this study, we aimed to identify DENV-2 subtypes in Aedes aegypti pools collected between 2011 and 2017 in a rural area of Northern Cordoba, Colombia ("La Balsa"). METHODS: RT-PCR was performed to analyze the capsid/pre-membrane region (C-PrM). Sequencing and phylogenetic bay...
Aedes/virología,
Teorema de Bayes,
Colombia/epidemiología,
Dengue/epidemiología,
Dengue/virología,
Virus del Dengue/clasificación,
Virus del Dengue/genética,
Virus del Dengue/aislamiento & purificación,
Mosquitos Vectores/virología,
Filogenia,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Serogrupo,
Serotipificación,
Dengue Grave
ABSTRACT Dengue, the most prevalent arboviral disease worldwide, is caused by any of the four dengue virus (DENV) serotypes that co-circulate constantly in hyperendemic areas such as Medellin (Colombia), and these serotypes are transmitted by mosquitoes of the genus Aedes. In this study, we evaluated the...
Aedes/virología,
Colombia,
Dengue/transmisión,
Dengue/virología,
Virus del Dengue/genética,
Virus del Dengue/aislamiento & purificación,
Virus del Dengue/fisiología,
Insectos Vectores/virología,
Filogenia,
Serogrupo,
Población Urbana,
Replicación Viral
Resumen Introducción: La viremia plasmática es un factor cuyo rol en la gravedad de la infección por el virus del dengue se ha discutido ampliamente en los últimos años, siendo hallados resultados divergentes en los múltiples contextos evaluados. Métodos: Se realizó una revisión sistemática d...
Dengue/diagnóstico,
Dengue/virología,
Virus del Dengue/aislamiento & purificación,
Carga Viral,
Viremia/virología,
Anticuerpos Antivirales,
Virus del Dengue/genética,
Genotipo,
Serogrupo,
Índice de Severidad de la Enfermedad,
Viremia/genética
