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Improving the thermostability of Trichoderma reesei xylanase 2 by introducing disulfide bonds

Tang, Feng; Chen, Daiwen; Yu, Bing; Luo, Yuheng; Zheng, Ping; Mao, Xiangbing; Yu, Jie; He, Jun.
Electron. j. biotechnol; 26 (), 2017
Background: Xylanases are considered one of the most important enzymes in many industries. However, their low thermostability hampers their applications in feed pelleting, pulp bleaching, and so on. The main aim of this work was to improve the thermostability of Trichoderma ressei xylanase 2 (Xyn2) by in...
Trichoderma/enzimología, Trichoderma/genética, Trichoderma/metabolismo, Estabilidad de Enzimas, Disulfuros/metabolismo, Mutación, Xilosidasas/metabolismo, Xilosidasas/genética, Concentración de Iones de Hidrógeno, Temperatura, Espectrometría de Masas, Cinética, Mutagénesis Sitio-Dirigida, Xilanos/metabolismo
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High levels of ß-xylosidase in Thermomyces lanuginosus: potential use for saccharification

Corrêa, Juliana Moço; Christi, Divair; Torre, Carla Lieko Della; Henn, Caroline; Conceição-Silva, José Luis da; Kadowaki, Marina Kimiko; Simão, Rita de Cássia Garcia.
Braz. j. microbiol; 47 (3), 2016
ABSTRACT A new strain of Thermomyces lanuginosus was isolated from the Atlantic Forest biome, and its β-xylosidases optimization in response to agro-industrial residues was performed. Using statistical approach as a strategy for optimization, the induction of β-xylosidases activity was evaluate...
Ascomicetos/enzimología, Fermentación, Xilosidasas/metabolismo, Biomasa, Activación Enzimática, Concentración de Iones de Hidrógeno, Hidrólisis, Polisacáridos/química, Polisacáridos/metabolismo, Especificidad por Sustrato, Temperatura, Xilosa/metabolismo, Zea mays/química
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Xylanase and beta-xylosidase from Penicillium janczewskii: Purification, characterization and hydrolysis of substrates

Fanchini Terrasan, César Rafael; Guisan, José Manuel; Cano Carmona, Eleonora.
Electron. j. biotechnol; 19 (5), 2016
Background: Xylanases and β-D-xylosidases are the most important enzymes responsible for the degradation of xylan, the second main constituent of plant cell walls. Results: In this study, the main extracellular xylanase (XYL I) and p-xylosidase (BXYL I) from the fungus Penicillium janczewskii were p...
Penicillium/enzimología, Xilosidasas/aislamiento & purificación, Xilosidasas/metabolismo, Hidrólisis, Electroforesis, Peso Molecular, Concentración de Iones de Hidrógeno, Temperatura, Estabilidad de Enzimas, Carbohidratos
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Production of enzymes from Lichtheimia ramosa using Brazilian savannah fruit wastes as substrate on solid state bioprocessess

Silva, Cinthia Aparecida de Andrade; Lacerda, Maria Priscila Franco; Leite, Rodrigo Simões Ribeiro; Fonseca, Gustavo Graciano.
Electron. j. biotechnol; 16 (5), 2013
Background: Enzyme production by solid state bioprocess (SSB) using residues as substrate for microorganisms is an alternative for costs reduction and to avoid their disposal into environment. The aim of this work was to evaluate the physiology of the fungus Lichtheimia ramosa in terms of microbial growt...
Mucorales/enzimología, Amilasas/metabolismo, Celulasas/metabolismo, Xilosidasas/metabolismo, Reactores Biológicos, Residuos, Frutas, Brasil, Mucorales/crecimiento & desarrollo
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