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RecET recombination system driving chromosomal target gene replacement in Zymomonas mobilis

Wu, Yan; Li, Tao; Cao, Qinghua; Li, Xuedan; Zhang, Yizheng; Tan, Xuemei.
Electron. j. biotechnol; 30 (), 2017
Background: Zymomonas mobilis is a Gram-negative microaerophilic bacterium with excellent ethanol-producing capabilities. The RecET recombination system provides an efficient tool for direct targeting of genes in the bacterial chromosome by PCR fragments. Results: The plasmids pSUZM2a-RecET and pSUZM2a-R...
Zymomonas/genética, Recombinación Homóloga, Alcohol Deshidrogenasa/metabolismo, Zymomonas/enzimología, Mutación, Etanol/metabolismo, Recombinación Genética, Técnicas de Inactivación de Genes, Electroporación, Plásmidos
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Three new shuttle vectors for heterologous expression in Zymomonas mobilis

Cao, Qinghua; Li, Tao; Shao, Huanhuan; Tan, Xuemei; Zhang, Yizheng.
Electron. j. biotechnol; 19 (1), 2016
Background: Zymomonas mobilis, as a novel platform for bio-ethanol production, has been attracted more attention and it is very important to construct vectors for the efficient expression of foreign genes in this bacterium. Results: Three shuttle vectors ( pSUZM 1, pSUZM2 and pSUZM3 ) were first construc...
Zymomonas/genética, Zymomonas/metabolismo, Expresión Génica/genética, Vectores Genéticos/genética, Glucano 1,4-alfa-Glucosidasa, Plásmidos, Reacción en Cadena en Tiempo Real de la Polimerasa, Fermentación
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