Triiodothyronine (T3) upregulates the expression of proto-oncogene TGFA independent of MAPK/ERK pathway activation in the human breast adenocarcinoma cell line, MCF7
Arch. endocrinol. metab. (Online); 63 (2), 2019
Publication year: 2019
ABSTRACT Objective:
To verify the physiological action of triiodothyronine T3 on the expression of transforming growth factor α (TGFA) mRNA in MCF7 cells by inhibition of RNA Polymerase II and the MAPK/ERK pathway Materials and methods: The cell line was treated with T3 at a physiological dose (10−9M) for 10 minutes, 1 and 4 hour (h) in the presence or absence of the inhibitors, α-amanitin (RNA polymerase II inhibitor) and PD98059 (MAPK/ERK pathway inhibitor). TGFA mRNA expression was analyzed by RT-PCR. For data analysis, we used ANOVA, complemented with the Tukey test and Student t-test, with a minimum significance of 5%.Results:
T3 increases the expression of TGFA mRNA in MCF7 cells in 4 h of treatment. Inhibition of RNA polymerase II modulates the effect of T3 treatment on the expression of TGFA in MCF7 cells. Activation of the MAPK/ERK pathway is not required for T3 to affect the expression of TGFA mRNA.Conclusion:
Treatment with a physiological concentration of T3 after RNA polymerase II inhibition altered the expression of TGFA. Inhibition of the MAPK/ERK pathway after T3 treatment does not interfere with the TGFA gene expression in a breast adenocarcinoma cell line.
Adenocarcinoma/genética, Adenocarcinoma/metabolismo, Neoplasias de la Mama/genética, Neoplasias de la Mama/metabolismo, Línea Celular Tumoral/metabolismo, Regulación Neoplásica de la Expresión Génica/genética, Sistema de Señalización de MAP Quinasas/genética, Células MCF-7/metabolismo, Proto-Oncogenes/genética, ARN Mensajero/genética, Factor de Crecimiento Transformador alfa/efectos de los fármacos, Factor de Crecimiento Transformador alfa/genética, Factor de Crecimiento Transformador alfa/metabolismo, Triyodotironina/genética, Triyodotironina/metabolismo, Triyodotironina/farmacología