Enhanced alkaline catalase production by Serratia marcescens FZSF01: enzyme purification, characterization, and recombinant expression
Electron. j. biotechnol; 30 (), 2017
Publication year: 2017
Background:
Catalase (CAT) is an important enzyme that degrades H2O2 into H2O and O2. To obtain an efficient catalase, in this study, a new strain of high catalase-producing Serratia marcescens, named FZSF01, was screened and its catalase was purified and characterized.Results:
After optimization of fermentation conditions, the yield of catalase produced by this strain was as high as 51,468 U/ml.This catalase was further purified using two steps:
DEAE-fast flow and Sephedex-G150. The purified catalase showed a specific activity of 197,575 U/mg with a molecular mass of 58 kDa. This catalase exhibited high activity at 20–70°C and pH 5.0–11.0. Km of the catalase was approximately 68 mM, and Vmax was 1886.8 mol/min mg. This catalase was further identified by LC–MS/MS, and the encoding gene was cloned and expressed in Escherichia coli BL21 (DE3) with a production of 17,267 ± 2037 U/ml.Conclusions:
To our knowledge, these results represent one of the highest fermentation levels reported among current catalase-producing strains. This FZSF01 catalase may be suitable for several industrial applications that comprise exposure to alkaline conditions and under a wide range of temperatures.
Serratia marcescens/enzimología, Serratia marcescens/genética, Catalasa/metabolismo, Catalasa/aislamiento & purificación, Catalasa/genética, Fermentación, Peróxido de Hidrógeno/metabolismo, ARN Ribosómico 16S, Análisis de Secuencia de ADN, Electroforesis, Cromatografía Liquida, Escherichia coli/genética, Escherichia coli/metabolismo, Recombinación Genética, Cinética