Evaluation of the commercial kit SIRE nitratase for detecting resistant mycobacterium tuberculosis in Brazil
Rev. Soc. Bras. Med. Trop; 50 (4), 2017
Publication year: 2017
Abstract INTRODUCTION:
This study aimed to evaluate a new commercial kit, Kit SIRE Nitratase-PlastLabor, for testing the drug susceptibility of clinical Mycobacterium tuberculosis isolates.METHODS:
The accuracy of the Kit SIRE Nitratase was evaluated by examining the susceptibility (streptomycin, isoniazid, rifampicin, and ethambutol) of 40 M. tuberculosis isolates, using the proportion method with Lowenstein-Jensen medium or the BACTEC MGIT 960 system.RESULTS:
The detection accuracy for streptomycin, isoniazid, rifampicin, and ethambutol was 95%, 97.5%, 100%, and 80%, respectively.CONCLUSIONS:
The exceptional accuracy demonstrated by Kit SIRE Nitratase for isoniazid and rifampicin makes the kit an attractive option for screening M. tuberculosis strain resistance.
Antibióticos Antituberculosos/farmacología, Pruebas Enzimáticas Clínicas/métodos, Farmacorresistencia Bacteriana, Etambutol/farmacología, Isoniazida/farmacología, Pruebas de Sensibilidad Microbiana/métodos, Mycobacterium tuberculosis/efectos de los fármacos, Mycobacterium tuberculosis/aislamiento & purificación, Oxidorreductasas/farmacología, Reproducibilidad de los Resultados, Rifampin/farmacología, Estreptomicina/farmacología