Development and characterization of a packaging cell line for pseudo-infectious yellow fever virus particle generation
Rev. Soc. Bras. Med. Trop; 51 (1), 2018
Publication year: 2018
Abstract INTRODUCTION:
Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins.METHODS:
HEK293 cells were transfected with YFV prM/E and C (84 nt) genes to generate HEK293-YFV-PrM/E-opt. The cells were evaluated for their ability to express the heterologous proteins and to package the replicon repYFV-17D-LucIRES, generating YFV-PIVs.RESULTS:
The expression of prM/E proteins was confirmed, and the cell line trans-packaged the replicon for recovery of a reporter for the YFV-PIVs.CONCLUSIONS:
HEK293-YFV-prM/E-opt trans-packaging capacity demonstrates its possible biotechnology application.
Citometría de Flujo, Técnica del Anticuerpo Fluorescente Indirecta, Proteínas Fluorescentes Verdes, Células HEK293, Vacunas de Partículas Similares a Virus/genética, Vacunas de Partículas Similares a Virus/inmunología, Ensamble de Virus/genética, Ensamble de Virus/inmunología, Replicación Viral/genética, Replicación Viral/inmunología, Virus de la Fiebre Amarilla/genética, Virus de la Fiebre Amarilla/inmunología