Clonal relation and antimicrobial resistance pattern of extended-spectrum β-lactamase- and AmpC β-lactamase-producing Enterobacter spp. isolated from different clinical samples in Tehran, Iran
Rev. Soc. Bras. Med. Trop; 51 (1), 2018
Publication year: 2018
Abstract INTRODUCTION:
Here, we determined the genes encoding antibiotic resistance enzymes and virulence factors and evaluated the genetic relationship between Enterobacter spp. isolated from different clinical samples.METHODS:
A total of 57 clinical isolates of Enterobacter spp. were tested for the production of extended-spectrum β-lactamases (ESBLs), carbapenemase, and AmpC using phenotypic and genotypic methods.RESULTS:
The most common ESBLs and AmpC β-lactamases were bla TEM (63.3%) and bla EBC (57.7%), respectively. The most prevalent virulence gene was rpos (87.7%). The random amplified polymorphic DNA (RAPD) patterns of strains were genetically unrelated.CONCLUSIONS:
RAPD polymerase chain reaction analysis revealed high genetic diversity among isolates.
Antibacterianos/farmacología, Proteínas Bacterianas/biosíntesis, Proteínas Bacterianas/efectos de los fármacos, Proteínas Bacterianas/genética, Células Clonales, Pruebas Antimicrobianas de Difusión por Disco, Farmacorresistencia Bacteriana Múltiple, Escherichia coli/efectos de los fármacos, Escherichia coli/enzimología, Escherichia coli/genética, Heces/microbiología, Genotipo, Irán, Fenotipo, Reacción en Cadena de la Polimerasa, beta-Lactamasas/biosíntesis, beta-Lactamas/efectos adversos, beta-Lactamasas/genética