IgE antibodies from schistosomiasis patients to recognize epitopes in potato apyrase
Rev. Soc. Bras. Med. Trop; 52 (), 2019
Publication year: 2019
Abstract INTRODUCTION:
High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins.METHODS:
Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity.RESULTS:
Most of the schistosomiasis patient's (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production.CONCLUSIONS:
Potato apyrase and SmATPDases have IgE-binding epitopes.
Anticuerpos Antihelmínticos/inmunología, Apirasa/inmunología, Western Blotting, Reacciones Cruzadas, Ensayo de Inmunoadsorción Enzimática, Epítopos/inmunología, Inmunoglobulina E/inmunología, Ratones Endogámicos C57BL, Schistosoma mansoni/inmunología, Esquistosomiasis mansoni/inmunología, Solanum tuberosum/enzimología