Influence of β-tricalcium phosphate of different geometric shape on the morphology of regeneration of experimental defect of compact bone tissue

Korenkov, Alexey

Purpose:

to compare the healing process of a defect of compact bone tissue after the implantation of osteoplastic materials based on β-tricalcium phosphate (“ChronOS™” and “Calc-i-oss®”), which differ by manufacturer, geometrical shape and microscopic structure.

Methods:

the experiment was performed on 48 white male Wistar rats. In the middle third of the diaphysis of the femur we produced a perforated defect of 2.5 mm diameter in the medullary canal, which in the animals of the first group was filled with the osteoplastic material “ChronOS™” (block, Synthes, Switzerland), and in the animals of the second group with “Calc-i-oss®” (granules, «Degradable Solutions Dental», Switzerland). Fragments of the injured bones were studied on the 60th and 120th day by light microscopy with morphometry and by scanning electron microscopy.

Results:

it was found that regardless the geometric shape and the microscopic structure, both osteoplastic materials show high biocompatibility, osteoconductive properties, good integration with bone tissue of the regenerate, and that the microscopic structure of β-tricalcium phosphate (“ChronOS™”) may significantly affect the microscopic structure of bone tissue of the regenerate, which manifests itself in the specificity of its geometric shape. It was noticed that osteoplastic materials “ChronOS™” and “Calc-i-oss®” almost at the same rate were subjected to resorption and replacement by the bone tissue, the ratio of which was 22.55±1.25 to 77.45±1.25 and 25.72±2.06% to 74.28±2.06% on the 60th day of the experiment, and 17.65±1.09 to 82.35±1.09 and 18.31±1.54% to 81.69±1.54% on the 120th day. (AU)

Objetivo:

Comparar el proceso de cicatrización de un defecto del tejido óseo compacto tras la implantación de materiales osteoplásticos a base de fosfato β-tricálcico («ChronOS™» and «Calc-i-oss®») que difieren según el fabricante en la forma geométrica y estructura microscópica. El estudio fue realizado en 48 ratas Wistar machos en los cuales se produjo, en el tercio medio de la diáfisis del fémur, un defecto perforado de 2,5 mm de diámetro, el cual fue llenado con el material «ChronOS™» (block, Synthes, Switzerland) en un grupo y con «Calc-i-oss®» (granules, «Degradable Solutions Dental», Switzerland) en el segundo grupo. El sector del defecto fue evaluado en los días 60 y 120 por microscopía óptica y por microscopía electrónica de barrido.

Resultados:

independientemente de la forma geométrica y la estructura microscópica, ambos materiales osteoplásticos mostraron alta biocompatibilidad, propiedades osteoconductivas y buena integración con el tejido óseo regenerado. La estructura microscópica del fosfato β-tricálcico («ChronOS™») puede afectar significativamente a la estructura microscópica del tejido óseo regenerado, que se manifiesta en su forma geométrica. Adicionalmente, se observó que ambos materiales osteoplásticos «ChronOS™» y «Calc-i-oss®» mostraron valores similares de resorción y reemplazo por tejido óseo, cuya relación al 60º día del experimento fue de 22,55±1,25 a 77,45±1,25 y 25,72±2,06% a 74,28±2,06%, y a los 120 días de 17,65±1,09 a 82,35±1,09 y de 18,31±1,54% a 81,69±1,54% respectivamente. (AU)