Identification of plasmid IncQ1 and NTEKPC-IId harboring bla KPC-2 in isolates from Klebsiella pneumoniae infections in patients from Recife-PE, Brazil
Rev. Soc. Bras. Med. Trop; 53 (), 2020
Publication year: 2020
Abstract INTRODUCTION:
This study investigated the genetic environment of bla KPC-2 in Klebsiella pnemoniae multi-drug resistant clinical isolates.METHODS:
Four carbapenemase gene isolates resistant to carbapenems, collected from infected patients from two hospitals in Brazil, were investigated using polymerase chain reaction and plasmid DNA sequencing.RESULTS:
The bla KPC-2 gene was located between ISKpn6 and a resolvase tnpR in the non-Tn4401 element (NTEKPC-IId). It was detected on a plasmid belonging to the IncQ1 group. CONCLUSIONS To our knowledge, this is the first report of the presence of the bla KPC-2 gene in the NTEKPC-IId element carried by plasmid IncQ1 from infections in Brazil.
Antibacterianos/farmacología, ADN Bacteriano/genética, Farmacorresistencia Bacteriana Múltiple, Infecciones por Klebsiella/microbiología, Klebsiella pneumoniae/enzimología, Klebsiella pneumoniae/genética, Klebsiella pneumoniae/aislamiento & purificación, Pruebas de Sensibilidad Microbiana, Plásmidos/genética, Reacción en Cadena de la Polimerasa, beta-Lactamasas/genética