Polifenoles, capacidad antioxidante y factor de protección solar de Borago officinalis L. “borraja” dispensada en las farmacias naturales de EsSalud
Polyphenols, antioxidant capacity and sun protection factor of Borago officinalis L. “borraja” dispensed in the Natural Pharmacies of EsSalud
Rev. peru. med. integr; 3 (3), 2018
Publication year: 2018
Objetivos. Cuantificar los polifenoles, la capacidad antioxidante y el factor de protección solar de las hojas y flores de Borago officinalis L. dispensadas en las farmacias naturales de EsSalud. Materiales y métodos. Se prepararon tres extractos fluidos en etanol (96%, 70% y 40%) y dos extractos acuosos (decocto e infuso), se concentraron bajo vacío hasta obtener extractos secos (ES). Los polifenoles fueron cuantificados utilizando el método analítico de Folin-Ciocalteu, dichos valores fueron expresados como equivalentes de ácido gálico (EAG), la capacidad antioxidante fue determinada por el método de DPPH (1,1-difenil-2- picrilhidracilo), expresada en equivalentes de ácido ascórbico (EAA), del mismo modo como equivalentes de butilhidroxitolueno (EBHT). El factor de protección solar (FPS) se evaluó por el método espectrofotométrico en el rango de 290 a 320 nm. Resultados. Se cuantificaron polifenoles en el rango de 157,487 ± 3,628 y 66,777 ± 0,734 mg EAG/g ES del extracto fluido de 70 y 96%, respectivamente; la capacidad antioxidante entre 198,631 ± 5,636 y 73,699 ± 5,946 mg EAA/g ES del infuso y decocto, respectivamente, así como 388,694 ± 10,591 y 153,919 ± 11,174 mg EBHT/g ES del infuso y decocto; el FPS en el rango de 8,91 ± 0,07 y 5,53 ± 0,02 para el extracto fluido de 40% e infuso, respectivamente. Conclusiones. El infuso de Borago officinalis L. dispensada en las farmacias naturales de EsSalud, reportó polifenoles con la mayor capacidad
antioxidante, y el extracto fluido de 40% presentó el mayor valor de FPS.
Objectives. Quantitation of polyphenols, antioxidant capacity and the sun protection factor of the leaves and flowers of Borago officinalis L. dispensed in Natural Pharmacies of EsSalud. Materials and methods. Three extracts were prepared with ethanol (96%, 70% and 40%) and two aqueous extracts (decoction and infusion), were removed volatile solvents under vacuum until obtaining dry extract (DE). Polyphenols were quantified by using analytic method referred as Folin-Ciocalteu, such values were expressed as gallic acid equivalents (GAE). On the other hand, the antioxidant capacity was determined by DPPH (1,1-diphenyl- 2-picrylhydracil) method, as well as values expressed in ascorbic acid equivalents (AAE) and butylated hydroxytoluene equivalents (BHTE). The sun protection factor values (SPF) were evaluated by using spectrophotometric method which values were in the range 290 to 320 nm. Results. Polyphenols were quantified in the range of 157.487 ± 3.628 and 66.777 ± 0.734 mg GAE/g DE of the redissolved and dilute extract of 70% and 96%, respectively. The antioxidant capacity was found between 198.631 ± 5.636 and 73.699 ± 5.946 mg AAE/g DE of the infusion and decoction, respectively, as well as 388.694 ± 10.591 and 153.919 ± 11.174 mg BHTE/g DE of the infusion and decoction; the SPF in the range 8.91 ± 0.07 and 5.53 ± 0.02 for the fluid extract of 40% and infusion, respectively. Conclusions. Infusion extract of Borago officinalis L. dispensed at the Natural Pharmacies of EsSalud, reported high concentration of polyphenols with the highest antioxidant activity. And the 40% redissolved and dilute extract was found a high SPF value.