This research study aimed at applying the morphometric quantification of the canine index for sexual dimorphism in a Brazilian sample. This was an observational study and the convenience sample consisted of 90 adult participants (45 male subjects and 45 female subjects), aging from 18 to 35 years. With the aidof a digital caliper, the intraoral mesiodistal measurement of the permanent mandibular right canine (MD43) and the intercanine distance (IC) were taken by three examiners. The measurements were applied to the mandibular canine index formula to estimate sex based on the morphometric features of human canines. The applicability of this approach for sexual dimorphism was assessed based on the mandibular canine index (MCI) calculated by the formula. The MCI was higher in male than in female subjects. In the total sample, the MCI overall mean accuracy rate for sexual dimorphism was 52.22%. In male subjects, the MCI was able to properly differentiate sex in 82.22% of the sample, while in female subjects the accuracy rate decreased to 22.22%. These results call the attention to the careful use of MCI especially for Forensic Anthropology. In particular, the accuracy of the method was close to the random of a sample that contained both sexes. Thus, the MCI should not be used as the only tool for sexual dimorphism.