Nine adult males of capuchin monkey (Cebus apella) were electro-ejaculated with a rectal bipolar probe under general anaesthesia with tiletamine -zolazepan association. From the obtained ejaculate, we only analysed the liquid fraction in order to avoid any kind of chemical treatment to attain dissolution of the seminal coagulum. The mean obtained volume was 0,2ml with the sperm concentration of 56.169 x 10(6) sperm/ml and mean motility 68,4 percent and vigor 2,6. The mean percentage of morphologic normal spermatozoa was 39 percent. The technique was efficient for semen collection in Cebus apella and allowed seminal evaluation without possible sperm lesions caused by chemical treatment for coagulum dissolution