Distinct protocols can be used to clean the hospital environment. This study used the ATP tool to evaluate cleaning protocols composed of either a quaternary ammonium compound plus biguanide (QACB) or bleach only. No statistical difference was found (p = 0.450) between QACB and bleach protocols, both being effective in hospital ward cleaning. So, it is possible to conclude that the use of QACB or bleach protocols ensures appropriate cleaning in health care, promoting a safe environment for patient use.