BACKGROUND Recently, nanomaterials have received much attention due to their important role in solving medical and environmental problems. In the present study, a novel nanocomposite based on mycosyn thesized bimetallic zinc-copper oxides nanoparticles, nanocellulose, and chitosan was ready through an ecofriendly method. Characterization, antimicrobial and photocatalytic activities were evaluated. RESULTS The result revealed that the prepared nanocomposite exhibited antibacterial activity against Bacillus subtilis, Escherichia coli and Staphylococcus aureus, where MICs were 7.81, 31.25 and 62.5 lg mL 1 . As well, the nanocomposite showed potential antifungal activity against Aspergillus brasiliensis where MIC was 7.81 lg mL 1 , but had minimal antifungal efficacy against Cryptococcus neo formans and Candida albicans where the MIC was 250 lg mL 1 for each other. Furthermore, the nanocom posite had photocatalytic activity. The bimetallic and nanocomposite materials were characterized via physiochemical and topographic analysis. CONCLUSIONS In conclusion, the prepared nanocomposite based on mycosynthesized bimetallic zinc copper oxides nanoparticles nanocellulose and chitosan has antimicrobial and photocatalytic activities which can be applied to various environments