Ultrastructural Aspects of Spermatids in Isogenic Black Mouse C57BL6J
Aspectos Ultraestructurales de las Espermátidas en el Ratón Negro Isogénico C57BL6
Int. j. morphol; 23 (4), 2005
Publication year: 2005
The objective of this study was to describe some changes in spermatids differentiating events during spermiogenesis, leading to spermatozoa final formation. The animals were sacrificed with ethylic acid inhalation and, then, testis fragments were collected, fixed in Karnovsky and post-fixed in osmium tetroxide. After dehydration, the sections were stained with uranyl acetate 2%. It was observed in early spermatids at Golgi phase, formation of acrosomal granule contained in the acrosomal vesicle, spheroidal nucleus with rugous reticulum around it, and Golgi apparatus made up of lamellae and vesicular structures. At cephalic hood phase, the granule suffers flattening and the marginal fossa is formed. In both phases, it was possible to observe the presence of multivesicular body. At acrosomal phase, the acrosomal system is set up, and several enzymes of endoplasmatic reticulum as well chromatoid body are observed in the cytoplasm. At the last phase, that is, maturation, the nucleus becomes more elongated, the endoplasmatic reticulum regresses, the Golgi apparatus approaches to the flagellum and mitochondrias align next to it.
El objetivo del estudio, fue describir algunas alteraciones producidas en la espermiogénesis, en los eventos que diferencian a las espermátidas y que llevan a la formación final de los espermatozoides. Los ratones fueron sacrificados con inhalación de éter etílico y, enseguida, se recolectaron fragmentos de testículos, los que fueron fijados en Karnovsky y tetróxido de osmio. Después de la deshidratación, los cortes fueron teñidos con acetato de uranilo al 2 %.