Estudio Estereológico del Hígado de Cobayo (Cavia porcellus)
Stereological Study of the Guinea-pig Liver (Cavia porcellus)
Int. j. morphol; 25 (3), 2007
Publication year: 2007
The liver is a widely researched organ for having an infinity of functions. On the other hand, Stereologyallows the access of quantitaive data of the study´s structure. Having access to this tpe of date of hepatocitos and sinusoids Under normal physiological conditions, it is necessary to determine how the number of these cells varies in pathological conditions. The principal objective of this investigation was to describe the morphology of the liver of cobayo, through Stereology, setting the bases for future morphofuncional investigations. Five adult males of the Cavia porcellus species were used, of which the liver was extracted and its volume determined, applying the Scherle method. Subsequently five pieces of aleatoria form were obtained which were set in an aqueous Bouin solution. Of these samples 3µm cuts were obtained, that were dyed with hematoxilina-eosina. For the stereological study the M42 system was used.
The following were determined:
the number densities, volume and surface and total number of hepatocitos and sinusoides. An index of lesser significance or equal to 0,05. The NV of hepatocitos was9.20 x 105 /mm³, V V de 70.04% and SV of 254.95 mm²/mm³. In the case of the sinusoides NV was 1.94 x 105 /mm³, V V of 24.86% and SV de 47.01 mm²/mm³. The total number of hepatocites was of 2.8 x 107 and sinusoides 0.61 x 107. While comparing the results obtained in Cavia porcellus and those obtained in other species, a quantitative variation in the hepatic morphology is observed. With these results it is possible to establish the bases for experimental research.
El hígado es un órgano ampliamente investigado por la infinidad de funciones que posee. Por otra parte, la Estereología permite obtener datos cuantitativos de la estructura en estudio. Tener acceso a este tipo de datos de los hepatocitos y sinusoides en condiciones fisiológicas normales, es necesario para determinar como varía el número de estas células en condiciones patológicas. El objetivo principal de esta investigación fue describir la morfología del hígado de cobayo a través de la Estereología, sentando las bases para futuras investigaciones morfofuncionales. Se utilizaron 5 machos adultos de la especie Cavia porcellus, a los cuales se les extrajo el hígado y se determinó su volumen aplicando el método de Scherle. Posteriormente, se obtuvieron 5 trozos de forma aleatoria, los que fueron fijados en solución de Bouin acuoso. De estas muestras se obtuvieron cortes de 3µm, que fueron teñidos con hematoxilina-eosina. Para el estudio estereológico, se usó el sistema M42.