CONTEXT:

Increased hydrogen peroxide has been described in the expired breath condensate (H2O2-E) of several lung conditions, such as acute respiratory distress syndrome, chronic obstructive pulmonary disease and asthma. This technique has been advocated as being a simple method for documenting airway inflammation.

OBJECTIVE:

To evaluate H2O2-E in healthy cigarette smokers, and to determine the acute effects of the consumption of one cigarette on H2O2-E levels.

TYPE OF STUDY:

Prospective, controlled trial.

SETTING:

A pulmonary function laboratory in a University Hospital.

PARTICIPANTS:

Two groups of healthy volunteers: individuals who had never smoked (NS; n=10; 4 men; age = 30.6 Ý 6.2 years) and current cigarette smokers (S; n=12; 7 men; age = 38.7 Ý 9.8). None of the volunteers had respiratory symptoms and all showed normal spirometric tests.

INTERVENTION:

Expired air was collected from all volunteers through a face mask and a plastic collecting system leading into a flask with dry ice and pure ethanol. Samples from the group S were collected twice, before and half an hour after the combustion of one cigarette.

MAIN MEASUREMENTS:

Expired hydrogen peroxide using the Gallati and Pracht method.

RESULTS:

The S and NS groups showed comparable levels of H2O2-E at basal conditions [NS = 0.74 muM (DP 0.24) vs. S = 0.75 muM (DP 0.31)]. The smokers showed a significant increase in H2O2-E levels half an hour after the consumption of only one cigarette [0.75 muM (DP 0.31) vs. 0.95 muM (DP 0.22)].

CONCLUSION:

The present results are consistent with the concept that smokers increase oxidative stress with elevated production of reactive oxygen species, contributing to the development of smoking-related disorders