Sera collected from 417 dairy cows from Londrina, PR, Brazil, were analysed by the indirect fluorescent antibody test (IFAT) for the presence of anitbody against Babesia bigemina, Babesia bovis e Anaplasma marginale.

All samples were tested diluted 1:

80 using rabbit anti-bovine IgG FITC conjugate diluted 1:100. Among the samples, 289 (67.30 per cent), 251 (60.19 per cent) and 281 (67.38 per cent) showed reactivity to B. bigemina, B. bovis and A. marginale, respectively. Although the number of reactive animals as relatively high, considerable percentages of negative animals (30.70 per cent, B. bigemina, 39.18 per cent, B. bovis and 32.62 per cent, A. marginale) were susceptible, characterizing the situation as enzootically instable