Swab samples obtained from 96 dogs with chronic otitis externa were cultured for the isolation of Staphylococcus species. Of 57 staphylococcal strains, 41 (72 per cent) were coagulase-negative (CNS). The identification of staphylococci strains was made by standard procedures for the routine identification of staphylococci in clinical practice. S. sciuri was the most frequent species isolated (22.8 per cent) from chronic otitis externa in dogs followed by S. intermedius (12.3 per cent), S. auricularis (10.5 per cent) and S. aureus (8.8 per cent). Three (5.2 per cent) CNS strains could not be identified. Bacterial isolates were susceptible to enrofloxacin, gentamicin, cephatothin, chloramphenicol and neomycin. Resistance was most common to penicillin G, oxacillin and ampicillin