Urethrostomy for serial sampling of urine was used due to the necessity of one technique that allowed the total urinary collection in definite time periods. Twenty-seven male Holstein calves from 8 to 16-days-age having a mean weight 39.50±4.80kg were used. Urethrostomy was carried out with the use of urethral sounding lead that was made for this purpose. In agreement with the clinical examinations, including seric biochemical, urinalysis and blood test and postmortem examination, the technique achieved 81.5 percent of satisfactory results.