Produção de embriões em ovinos Morada Nova e Somalis Brasileira
Embryo production in Morada Nova and Somalis Brasileira ewes
Arq. bras. med. vet. zootec; 68 (5), 2016
Publication year: 2016
Superovulatory response and embryo yield in 19 Morada Nova and 20 Somalis Brasileira ewes was
analyzed. All animals were synchronized with the insertion of an intravaginal device (CIDR®) on Day 0,
replaced by a new device on Day 7, which remained in place until Day 14 and superovulated with 133mg
of porcine FSH (pFSH) in decreasing doses at 12h intervals from Day 12 until Day 15 of the treatment,
and a single dose of equine chorionic gonadotropin (eCG, 200UI) on Day 14 (i.e., administered in CIDR
removal). Fifty hours after CIDR® removal, females were inseminated by laparoscopy. All embryos were
recovered by laparotomy 5 days after insemination. Sheep which responded to the superovulation
protocol (P>0.05) included 74% of the Morada Nova ewes and 50% of the Somalis Brasileira ewes.
Morada Nova showed better results (P<0.05) than Somalis Brasileira in number of ovulations (15.38 ±
5.24 vs. 10.56 ± 2.83), total structures (11.00 ± 7.55 vs. 3.33 ± 1.94) and embryo yields (6.79 ± 5.35 vs.
2.90 ± 2.18). Despite the high fertilization rate, degenerate embryo rate was high for both breeds, with an
overall rate of 39% (57/145). In conclusion, superovulatory response and embryo yields in Morada Nova
ewes were considered sufficient to justify the use of this procedure in genetic resources conservation
programs. However, improvements to embryo quality and control of precocious regression of corpus
luteum are necessary to produce better results in the MOET program, with minimal variations and
maximum embryo yield in Morada Nova and Somalis Brasileira ewes.(AU)