Alternative animal model for studies of total skin thickness burns

Acta cir. bras; 32 (10), 2017
Publication year: 2017

Abstract Purpose:

To present an alternative experimental model of third degree burn of easy reproducibility.

Methods:

Eighteen male Wister rats were randomly divided into three groups, 6 of which were allocated to each group. A soldering iron coupled to an aluminum plate was used to produce burn, at a temperature of 150ºC, with different exposure times per group. Group 5 (G5) animals were burned at 150°C with exposure time of 5 seconds; Group 10 (G10) the animals were burned at 150°C with exposure time of 10 seconds and group 15 (G15) the animals were burned at 150°C with exposure time of 15 seconds.

Results:

Histopathological analyzes showed that all three groups had similar morphological characteristics, with total thickness involvement.

Conclusion:

The technique is effective to reproduce a third degree burn and suggests the temperature of 150ºC with 5 seconds of exposure in order to minimize the risks to the animals.

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