Homology modeling and epitope prediction of Der f 33
Braz. j. med. biol. res; 51 (5), 2018
Publication year: 2018
Dermatophagoides farinae (Der f), one of the main species of house dust mites, produces more than 30 allergens. A recently identified allergen belonging to the alpha-tubulin protein family, Der f 33, has not been characterized in detail. In this study, we used bioinformatics tools to construct the secondary and tertiary structures and predict the B and T cell epitopes of Der f 33. First, protein attribution, protein patterns, and physicochemical properties were predicted. Then, a reasonable tertiary structure was constructed by homology modeling. In addition, six B cell epitopes (amino acid positions 34-45, 63-67, 103-108, 224-230, 308-316, and 365-377) and four T cell epitopes (positions 178-186, 241-249, 335-343, and 402-410) were predicted. These results established a theoretical basis for further studies and eventual epitope-based vaccine design against Der f 33.
Alérgenos/química, Alérgenos/genética, Alérgenos/inmunología, Antígenos Dermatofagoides/química, Antígenos Dermatofagoides/genética, Antígenos Dermatofagoides/inmunología, Biología Computacional, Dermatophagoides farinae/química, Dermatophagoides farinae/genética, Dermatophagoides farinae/inmunología, Mapeo Epitopo, Epítopos de Linfocito B/química, Epítopos de Linfocito B/genética, Epítopos de Linfocito T/química, Epítopos de Linfocito T/genética, Estructura Molecular, Estructura Terciaria de Proteína, Análisis de Secuencia de Proteína, Tubulina (Proteína)/química, Tubulina (Proteína)/genética, Tubulina (Proteína)/inmunología