Electroacupuncture ameliorate learning and memory by improving N -acetylaspartate and glutamate metabolism in APP/PS1 mice
Biol. Res; 51 (), 2018
Publication year: 2018
OBJECTIVE:
To explore the precise mechanism of electroacupuncture (EA) to delay cognitive impairment in Alzheimer disease. Methods N -Acetylaspartate (NAA), glutamate (Glu) and myoinositol (mI) metabolism were measured by magnetic resonance spectroscopy, learning and memory of APP/PS1 mouse was evaluated by the Morris water maze test and the step-down avoidance test, neuron survival number and neuronal structure in the hippocampus were observed by Nissl staining, and BDNF and phosphorylated TrkB detected by Western blot.RESULTS:
EA at DU20 acupuncture significantly improve learning and memory in behavioral tests, up-regulate NAA, Glu and mI metabolism, increase the surviving neurons in hippocampus, and promote the expression of BDNF and TrkB in the APP/PS1 transgenic mice.CONCLUSION:
These findings suggested that EA is a potential therapeutic for ameliorate cognitive dysfunction, and it might be due to EA could improve NAA and Glu metabolism by upregulation of BDNF in APP/PS1 mice.
Ácido Aspártico/análogos & derivados, Electroacupuntura/métodos, Ácido Glutámico/metabolismo, Hipocampo/química, Aprendizaje/fisiología, Memoria/fisiología, Ácido Aspártico/metabolismo, Hipocampo/diagnóstico por imagen, Inositol/análisis, Glicoproteínas de Membrana/análisis, Proteínas Tirosina Quinasas/análisis, Western Blotting, Factor Neurotrófico Derivado del Encéfalo, Prueba de Esfuerzo, Imagen por Resonancia Magnética, Espectroscopía de Resonancia Magnética, Aprendizaje por Laberinto, Ratones Transgénicos, Modelos Animales, Distribución Aleatoria