Avaliação in vitro da atividade de óleos essenciais sobre Candida auris
Publication year: 2021
Theses and dissertations in Portugués presented to the Coordenadoria de Controle de Doenças. Programa de Pós-graduação em Ciências to obtain the academic title of Doutor. Leader: Maria de Fátima Costa Pires
O objetivo deste trabalho foi avaliar a atividade in vitro dos óleos essenciais de Cinnamomum cassia, Eucalyptus citriodora; Eucalyptus globulus, Eugenia caryophyllus, Melaleuca alternifólia, Myristica fragans, Ocimum basilicum, Origanum majorana, Origanum vulgare, Rosamarinus officinalis, Salvia sclarea, Schinus terebinthifolius, Thymus vulgaris e Zingiber officinale, bem como dos compostos químicos desses óleos essenciais, com as menores concentrações fungicidas mínimas sobre três isolados biológicos de Candida auris de origem humana. Determinar e avaliar in vitro as concentrações fungicidas mínimas dos antifúngicos sintéticos anfotericina B, cetoconazol, flucitocina, fluconazol, itraconazol e voriconazol sobre Candida auris. Para avaliação da atividade antifúngica e obtenção da concentração fungicida
mínima (CFM) utilizou-se a técnica de microdiluição em caldo, na base 2, em meio RPMI 1640, acrescido de tensoativo Tween20, e a confirmação da inibição em Agar Sabouraud dextrose, a 37ºC por 24hs. Para a avaliação da sensibilidade a anfotericina B, cetoconazol, flucitocina...(AU)
The objective of this search was to evaluate the in vitro activity of the essential oils Cinnamomum cassia, Eucalyptus citriodora; Eucalyptus globulus, Eugenia caryophyllus, Melaleuca alternifolia, Myristica fragans, Ocimum basilicum, Origanum majorana, Origanum vulgare, Rosamarinus officinalis, Salvia sclarea, Schinus terebinthifolius, Thymus vulgaris and Zingiber officinale as well as the chemical compounds of these essencial oils with the lowest minimum fungicidal concentrations on three biological isolates of Candida auris of human origin. Determine and evaluate in vitro the minimum fungicidal concentrations of the synthetic antifungals amphotericin B, ketoconazole, flucitocin, fluconazole, itraconazole and voriconazole on Candida auris. To evaluate the antifungal activity and obtain the minimum fungicidal concentration (CFM), the broth microdilution technique was used in RPMI
1640 medium, plus Tween20 surfactant, and confirmation of inhibition in Sabouraud dextrose Agar, at 37ºC for 24 hours. For the evaluation of sensitivity to amphotericin B, ketoconazole, flucitocin, fluconazole, itraconazole and voriconazole, the commercial test E-test® was used. The three isolates were resistant to the oils Myristica fragans, Shinus
terebinthifolius and Zenziber officinale. The other essential oils showed inhibitory activity on Candida auris. The three isolates were more sensitive to Cinnamomum cassia essential oil, with CFM of 0.00001 µg/mL for isolate 1 and CFM of 0.10 µg/mL for isolate 2 and CFM of 0.05 µg/mL for isolate 3. Thymus vulgaris also showed lower fungicidal concentration for isolates 1 and 2, with CFM results of 684.76 µg/mL for isolate 1 and CFM of 171.19 µg/mL
for isolate 2. The three biological isolates evaluated were more sensitive to cinnamic aldehyde, isoeugenol and timol, with the following results being found for isolates 1, 2 and 3 respectively: Cinnamic aldehyde - CFM 0.026µg/mL, 0.83 µg/mL and 3, 33 µg/ml; Isoeugenol CFM of 1.60 µg/ml, 1.60 µg/ml and 12.82 µg/ml; timol CFM of 2.93 µg/ml, 1.46 µg/ml and 11.74 µg/ml.