Mayor, Alfredo;
Silva, Clemente;
Valbona, Eduard Rovira;
Feltrer, Arantxa Roca;
Bonnington, Craig;
Smith, Alexandra Wharton;
Greehouse, Bryan;
Bever, Caitlin;
Chidimatembue, Arlindo;
Guinovart, Caterina;
Proctor, Joshua L;
Rodrigues, Maria;
Canana, Neide;
Arnaldo, Paulo;
Boene, Simone;
Enosse, Sonia;
Saute, Francisco;
Candrinho, Baltazar.
Introduction: Genomic data constitute a valuable adjunct to routine surveillance that can guide programmatic decisions to reduce the burden of infectious diseases. However, genomic capacities remain low in Africa. This study aims to operationalise a functional malaria molecular surveillance system in Moz...
Background: Due to the threat of emerging anti-malarial resistance, the World Health Organization recommends incorporating surveillance for molecular markers of anti-malarial resistance into routine therapeutic efficacy studies (TESs). In 2018, a TES of artemether-lumefantrine (AL) and artesunate-amodiaq...
Antimalarials/pharmacology,
Antimalarials/therapeutic use,
Artemisinins/pharmacology,
Artemisinins/therapeutic use,
Drug Resistance/genetics,
Drug Therapy, Combination,
Genetic Markers,
Plasmodium falciparum/radiation effects,
Plasmodium falciparum/genetics,
Plasmodium falciparum/isolation & purification,
Polymorphism, Genetic/genetics,
Mozambique
Galatas, Beatriz;
Mayor, Alfredo;
Gupta, Himanshu;
Balanza, Núria;
Jang, Ihn Kyung;
Nhamussua, Lidia;
Simone, Wilson;
Cistero, Pau;
Chidimatembue, Arlindo;
Munguambe, Humberto;
Saute, Francisco;
Aide, Pedro;
Bassat, Quique.
An ultrasensitive malaria rapid diagnostic test (RDT) was recently developed for the improved detection of low-density Plasmodium falciparum infections. This study aimed to compare the diagnostic performance of the PfHRP2-based Abbott Malaria Ag P. falciparum ultrasensitive RDT (uRDT) to that of the conv...
Antigens, Protozoan/genetics,
Parasitology/methods,
d0077700,
d0126800,
Mozambique,
Antigens, Protozoan/blood,
Cross-Sectional Studies,
Dried Blood Spot Testing/methods,
Dried Blood Spot Testing/statistics & numerical data,
Malaria, Falciparum/diagnosis,
Parasitology/statistics & numerical data,
Plasmodium falciparum/genetics
Gupta, himanshu;
Bassat, quique;
Nhamussua, lidia;
Matambisso, gloria;
Cisteró, pau;
Huijben, silvie;
Chidimatembue, arlindo;
Galatas, beatriz;
Ménard, didier;
Ringwald, pascal;
Rabinovich, regina;
Alonso, pedro L;
Saute, francisco;
Assessor, pedro;
Prefeito, alfredo.
Background Mass drug administration (MDA) can rapidly reduce the burden of Plasmodium falciparum (Pf). However, concerns remain about its contribution to select for antimalarial drug resistance. Methods We used Sanger sequencing and real-time PCR to determine the proportion of molecular markers associate...
Antimalarials/administration & dosage,
Antimalarials/pharmacology,
Antimalarials/therapeutic use,
Artemisinins/administration & dosage,
Artemisinins/pharmacology,
Artemisinins/therapeutic use,
Antineoplastic Combined Chemotherapy Protocols,
Drug Resistance/genetics,
Malaria/parasitology,
Malaria/prevention & control,
Mozambique,
Plasmodium falciparum/immunology,
Plasmodium falciparum/genetics,
Plasmodium falciparum/pathogenicity,
Polymorphism, Genetic,
Protozoan Proteins/genetics,
Protozoan Proteins/metabolism,
Quinolines/administration & dosage,
Quinolines/therapeutic use,
Quinolines/pharmacology
Gupta, himanshu;
Galatas, beatriz;
Matambisso, gloria;
Nhamussua, lidia;
Cisteró, pau;
Bassat, quique;
Casellas, aina;
Macete, eusebio;
Aponte, john, j;
Sacoor, charfudin;
Alonso, pedro;
Saúte, francisco;
Guinovart, caterina;
Aide, pedro;
Mayor, alfredo.
Background: Poor knowledge on the afebrile Plasmodium falciparum biology limits elimination approaches to target asymptomatic malaria. Therefore, the association of parasite factors involved in cytoadhesion, parasite multiplication and gametocyte maturation with afebrile malaria was assessed.
Methods: Pl...
Gupta, himanshu;
Galatas, beatriz;
Matambisso, gloria;
Nhamussua, lidia;
Cisteró, pau;
Bassat, quique;
Casellas, aina;
Macete, eusébio;
Aponte, john J;
Sacoor, charfudin;
Alonso, pedro;
Saúte, francisco;
Guinovart, caterina;
Aide, pedro;
Mayor, alfredo.
Background: Poor knowledge on the afebrile Plasmodium falciparum biology limits elimination approaches to target asymptomatic malaria. Therefore, the association of parasite factors involved in cytoadhesion, parasite multiplication and gametocyte maturation with afebrile malaria was assessed.
Methods: Pl...
Malaria, Falciparum,
Plasmodium falciparum,
Protozoan Proteins,
Mozambique,
Malaria, Falciparum/diagnosis,
Malaria, Falciparum/parasitology,
Plasmodium falciparum/genetics,
Protozoan Proteins/metabolism,
Protozoan Proteins/genetics
Salvador, Crizolgo;
Rafael, Bernardete;
Matsinhe, Francisco;
Matsinhe, Francisco;
Candrinho , Baltazar;
Muthemba , Rosália;
Carvalho, Eva;
Naueia, Eva;
Sacarlal, Jahit;
Namboze, Josephine;
Tiago , Armindo;
Warsame, Marian;
Enosse, Sónia.
he resistance of Plasmodium falciparum to anti-malarial drugs continues to challenge malaria control. We assessed the therapeutic efficacy and safety of artemether-lumefantrine (AL), the first-line treatment of uncomplicated P. falciparum malaria, in children under five years of age in Mozambique. We con...
Antimalarials/therapeutic use,
Artemisinins/therapeutic use,
Malaria/epidemiology,
d0109630,
d0200660,
d0161330,
Artemether, Lumefantrine Drug Combination,
Drug Combinations,
Ethanolamines/therapeutic use,
Fluorenes/therapeutic use,
Malaria, Falciparum/therapy,
Malaria, Falciparum/epidemiology,
Malaria, Falciparum/parasitology,
Plasmodium falciparum/genetics,
Polymerase Chain Reaction,
Mozambique/epidemiology
Aguilar, Ruth;
Tejada, Ariel Magallon;
Achtman, Ariel H;
Moraleda, Cinta;
Joice, Regina;
Cisteró, Pau;
Suen, Connie S. N. Li Wai;
Nhabomba, Augusto;
Macete, Eusebio;
Mueller, Ivo;
Marti, Matthias;
Alonso, Pedro L;
Menéndez, Clara;
Schofield, Louis;
Mayor, Alfredo.
Plasmodium falciparum immature gametocytes are not observed in peripheral blood. However, gametocyte stages in organs such as bone marrow have never been assessed by molecular techniques, which are more sensitive than optical microscopy. We quantified P falciparum sexual stages in bone marrow (n = 174) a...
Anemia/parasitology,
Anemia/genetics,
Bone Marrow/pathology,
Bone Marrow/parasitology,
DNA, Protozoan/analysis,
Life Cycle Stages/genetics,
Malaria, Falciparum/diagnosis,
Malaria, Falciparum/genetics,
Malaria, Falciparum/parasitology,
Molecular Diagnostic Techniques,
Plasmodium falciparum/genetics,
Plasmodium falciparum/growth & development,
Plasmodium falciparum/isolation & purification,
Polymerase Chain Reaction,
Mozambique
Mayor, alfredo;
Casas, elisa serra;
Bardají, azucena;
Sanz, sergi;
Puyol, laura;
Cisteró, pau;
Sigauque, betuel;
Mandomando, inacio;
Aponte, john J;
Alonso, pedro L;
Menéndez, clara.
Background: Control of malaria in pregnancy remains a public health challenge. Improvements in its correct diagnosis and the adequacy of protocols to evaluate anti-malarial drug efficacy in pregnancy, are essential to achieve this goal.
Methods: The presence of Plasmodium falciparum was assessed by real-...
Malaria, Falciparum/epidemiology,
Merozoite Surface Protein 1,
Plasmodium falciparum,
Protozoan Proteins,
Pyrimethamine,
Malaria, Falciparum/therapy,
Malaria, Falciparum/parasitology,
Parasitemia/therapy,
Parasitemia/epidemiology,
Plasmodium falciparum/drug effects,
Plasmodium falciparum/genetics,
Plasmodium falciparum/isolation & purification,
Polymerase Chain Reaction,
Pregnancy Complications, Parasitic/therapy,
Pregnancy Complications, Parasitic/epidemiology,
Pregnancy Complications, Parasitic/parasitology
Background: Falciparum malaria affects pregnant women, especially primigravidae, but before malaria control programmes targeted to them can be designed, a description of the frequency and parity pattern of the infection is needed. There is little information on the frequency and effect of submicroscopic ...
