The flow cytometers that are currently supported by industry provide accurate CD4(+)-T-cell counts for monitoring human immunodeficiency virus disease but remain unaffordable for routine service work under resource-poor conditions. We therefore combined volumetric flow cytometry (measuring absolute lymph...
Introdução: Moçambique é um dos países endémicos à malária. Em 2011, a estimativa de prevalência desta doença era de 40–80% em crianças dos 2–9 anos e 90% em crianças menores de 5 anos. Estas altas prevalências podem ser devido à dificuldade das crianças em desenvolverem uma resposta i...
Laboratórios clínicos desempenham um papel importante no apoio ao sistema do Serviço Nacional de Saúde, proporcionando saúde de qualidade para a população moçambicana. Como parte deste sistema, os laboratórios trabalham lado a lado com outros sectores para garantir testagem apropriada para o dia...
Background: The acute phase of HIV infection is characterized by massive depletion of CD4 T cells, high viral plasma levels and pronounced systemic immune activation. Regulatory T cells (Tregs) have the potential to control systemic immune activation but also to suppress antigen specific T and B cell res...
Background: Measuring CD4 T-cell counts at low cost is relevant in dealing with the human immunodeficiency virus (HIV) epidemic throughout the developing world. The recently introduced novel concepts in gating strategies and sample stabilization facilitate affordable immunophenotyping by flow cytometry. ...
Background: North American and European guidelines for dual-platform (DP) flow cytometry recommendabsolute CD4 T-cell counts to be calculated from two parameters: the absolute lymphocyte counts obtainedon a hematology analyzer and the percentages of CD4 cells among lymphocytes (CD4%/lympho) obtainedby fl...
