Background: Malaria is still one of the leading causes of mortality and morbidity in Mozambique with little progress in malaria control over the past 20 years. Sussundenga is one of most affected areas. Malaria transmission has a strong association with environmental and sociodemographic factors. The kno...
Smith, Alexandra Wharton;
Baker, Kevin;
Feltrer, Arantxa Roca;
Rodrigues, Maria;
Richardson, Sol;
Bonnington, Craig A;
Rassi, Christian;
Marasciulo, Madeleine;
Enosse, Sonia;
Saute, Francisco;
Aide, Pedro;
Macete, Eusebio;
Candrinho, Baltazar.
Background
Malaria is a significant cause of morbidity and mortality in children aged under 5 years in Mozambique. The World Health Organization recommends seasonal malaria chemoprevention (SMC), the administration of four monthly courses of sulfadoxine-pyrimethamine (SP) and amodiaquine (AQ), to childre...
Galatas, Beatriz;
Mayor, Alfredo;
Gupta, Himanshu;
Balanza, Núria;
Jang, Ihn Kyung;
Nhamussua, Lidia;
Simone, Wilson;
Cistero, Pau;
Chidimatembue, Arlindo;
Munguambe, Humberto;
Saute, Francisco;
Aide, Pedro;
Bassat, Quique.
An ultrasensitive malaria rapid diagnostic test (RDT) was recently developed for the improved detection of low-density Plasmodium falciparum infections. This study aimed to compare the diagnostic performance of the PfHRP2-based Abbott Malaria Ag P. falciparum ultrasensitive RDT (uRDT) to that of the conv...
Antigens, Protozoan/genetics,
Parasitology/methods,
d0077700,
d0126800,
Mozambique,
Antigens, Protozoan/blood,
Cross-Sectional Studies,
Dried Blood Spot Testing/methods,
Dried Blood Spot Testing/statistics & numerical data,
Malaria, Falciparum/diagnosis,
Parasitology/statistics & numerical data,
Plasmodium falciparum/genetics
Em Moçambique a malária é responsável por 60% de todas as admissões pediátricas, por 40% do total de consultas e ainda por 30% de todas as mortes registadas nos hospitais (2).Com vista a reverter esse cenário, o governo moçambicano, por intermédio do Programa Nacional de Combate à Malária (PCN...
Malaria/epidemiology,
Malaria, Avian,
Mozambique/epidemiology,
Artemether/therapeutic use,
Artemether/supply & distribution,
Lumefantrine/therapeutic use,
Lumefantrine/supply & distribution,
Medical Care/methods,
Malaria, Falciparum/diagnosis,
Malaria, Falciparum/drug therapy,
Malaria, Falciparum/prevention & control,
Malaria, Falciparum/transmission,
Anopheles/virology
Gupta, himanshu;
Galatas, beatriz;
Matambisso, gloria;
Nhamussua, lidia;
Cisteró, pau;
Bassat, quique;
Casellas, aina;
Macete, eusebio;
Aponte, john, j;
Sacoor, charfudin;
Alonso, pedro;
Saúte, francisco;
Guinovart, caterina;
Aide, pedro;
Mayor, alfredo.
Background: Poor knowledge on the afebrile Plasmodium falciparum biology limits elimination approaches to target asymptomatic malaria. Therefore, the association of parasite factors involved in cytoadhesion, parasite multiplication and gametocyte maturation with afebrile malaria was assessed.
Methods: Pl...
Gupta, himanshu;
Galatas, beatriz;
Matambisso, gloria;
Nhamussua, lidia;
Cisteró, pau;
Bassat, quique;
Casellas, aina;
Macete, eusébio;
Aponte, john J;
Sacoor, charfudin;
Alonso, pedro;
Saúte, francisco;
Guinovart, caterina;
Aide, pedro;
Mayor, alfredo.
Background: Poor knowledge on the afebrile Plasmodium falciparum biology limits elimination approaches to target asymptomatic malaria. Therefore, the association of parasite factors involved in cytoadhesion, parasite multiplication and gametocyte maturation with afebrile malaria was assessed.
Methods: Pl...
Malaria, Falciparum,
Plasmodium falciparum,
Protozoan Proteins,
Mozambique,
Malaria, Falciparum/diagnosis,
Malaria, Falciparum/parasitology,
Plasmodium falciparum/genetics,
Protozoan Proteins/metabolism,
Protozoan Proteins/genetics
Mayor, alfredo;
Dobaño, carlota;
Nhabomba, augusto;
Guinovart, caterina;
Jiménez, alfonso;
Manaca, maria nelia;
Aguilar, ruth;
Barbosa, arnoldo;
Rodríguez, mauricio H;
Cisteró, pau;
Quimice, lazaro M;
Menéndez, clara;
Aponte, john J;
Ordi, jaume;
Chitnis, chetan E;
Alonso, pedro L.
Background: Difficulties to disentangle the protective versus exposure role of anti-malarial antibodies hamper the identification of clinically-relevant immune targets. Here, factors affecting maternal IgG and IgMs against Plasmodium falciparum antigens, as well as their relationship with parasite infect...
Malaria, Falciparum/diagnosis,
Mozambique,
Plasmodium falciparum/immunology,
Antibodies, Monoclonal,
Immunoglobulin G/metabolism,
Population Characteristics,
Malaria, Falciparum/epidemiology,
Malaria, Falciparum/prevention & control,
Pregnancy Complications, Parasitic/diagnosis,
Pregnancy Complications, Parasitic/epidemiology,
Pregnancy Complications, Parasitic/prevention & control,
Pregnancy Complications, Parasitic/parasitology,
Prevalence
Gupta, himanshu;
Matambisso, gloria;
Galatas, beatriz;
Simone, wilson;
Nhamussua, lidia;
Cisteró, pau;
Cunningham, jane;
Rabinovich, N regina;
Saute, francisco;
Alonso, pedro L;
Aide, pedro;
Mayor, Alfredo.
Background: Malaria programmes use Plasmodium falciparum histidine-rich protein-2 (PfHRP2) based rapid diagnos tic tests (RDTs) for malaria diagnosis. The deletion of this target antigen could potentially lead to misdiagnosis, delayed treatment and continuation of active transmission. Methods: Plasmodium...
Fonseca, ana maria;
Quinto, llorenç;
Jiménez, alfons;
González, raquel;
Bardají, azucena;
Maculuve, sonia;
Dobaño, carlota;
Rupérez, maria;
Vala, anifa;
Aponte, john, j;
Sevene, esperanza;
Macete, Eusebio;
Menéndez, clara;
Mayor, alfredo.
Background: Pregnant women exposed to Plasmodium falciparum generate antibodies against VAR2CSA, the parasite protein that mediates adhesion of infected erythrocytes to the placenta. There is a need of high-throughput tools to determine the fine specificity of these antibodies that can be used to identif...
Antibodies, Protozoan/blood,
Antibodies, Protozoan/immunology,
Antigens, Protozoan/genetics,
Antigens, Protozoan/immunology,
Antigens, Protozoan/metabolism,
Dried Blood Spot Testing,
Erythrocytes/parasitology,
Immunoassay,
Immunoglobulin G/blood,
Malaria, Falciparum/diagnosis,
Plasmodium falciparum/isolation & purification,
Pregnancy
Aguilar, Ruth;
Tejada, Ariel Magallon;
Achtman, Ariel H;
Moraleda, Cinta;
Joice, Regina;
Cisteró, Pau;
Suen, Connie S. N. Li Wai;
Nhabomba, Augusto;
Macete, Eusebio;
Mueller, Ivo;
Marti, Matthias;
Alonso, Pedro L;
Menéndez, Clara;
Schofield, Louis;
Mayor, Alfredo.
Plasmodium falciparum immature gametocytes are not observed in peripheral blood. However, gametocyte stages in organs such as bone marrow have never been assessed by molecular techniques, which are more sensitive than optical microscopy. We quantified P falciparum sexual stages in bone marrow (n = 174) a...
Anemia/parasitology,
Anemia/genetics,
Bone Marrow/pathology,
Bone Marrow/parasitology,
DNA, Protozoan/analysis,
Life Cycle Stages/genetics,
Malaria, Falciparum/diagnosis,
Malaria, Falciparum/genetics,
Malaria, Falciparum/parasitology,
Molecular Diagnostic Techniques,
Plasmodium falciparum/genetics,
Plasmodium falciparum/growth & development,
Plasmodium falciparum/isolation & purification,
Polymerase Chain Reaction,
Mozambique
