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Precise CD4 T-cell counting using red diode laser excitation: for richer, for poorer

Janossy, george; Jani, ilesh, v; Kahan, melvyn; Barnett, david; Mandy, frank; Shapiro, howard.
Cytometry, Part A; 50 (2), 2002
Background: Measuring CD4 T-cell counts at low cost is relevant in dealing with the human immunodeficiency virus (HIV) epidemic throughout the developing world. The recently introduced novel concepts in gating strategies and sample stabilization facilitate affordable immunophenotyping by flow cytometry. ...
Antígenos CD4/biossíntese, Linfócitos T CD4-Positivos/citologia, Citometria de Fluxo, Soropositividade para HIV/sangue, Contagem de Células Sanguíneas, Citometria de Fluxo/economia, Imunofenotipagem/métodos, Imunofenotipagem/economia, Citometria de Fluxo/instrumentação, Imunofenotipagem/instrumentação, Espalhamento de Radiação, Lasers, Moçambique
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CD45-assisted PanLeucogating for accurate, cost-effective dual-platform CD4+ T-cell enumeration

Glencross, deborah; Scott, lesley, e; Jani, jlesh, v; Barnett, david; Janossy, george.
Cytometry, Part A; 50 (2), 2002
Background: North American and European guidelines for dual-platform (DP) flow cytometry recommendabsolute CD4 T-cell counts to be calculated from two parameters: the absolute lymphocyte counts obtainedon a hematology analyzer and the percentages of CD4 cells among lymphocytes (CD4%/lympho) obtainedby fl...
Linfócitos T CD4-Positivos/citologia, Citometria de Fluxo/economia, Citometria de Fluxo/métodos, Citometria de Fluxo/instrumentação, Imunofenotipagem/instrumentação, Imunofenotipagem/economia, Antígenos Comuns de Leucócito/biossíntese, Controle de Qualidade
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