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Affordable CD4(+)-T-cell counting by flow cytometry: CD45 gating for volumetric analysis

Janossy, george; Jani, Ilesh, v; Bradley, nicholas, j; Bikoue, arsene; Pitfield, tim; Glencross, debbie, k.
Clin. diagn. lab. immunol; 9 (5), 2022
The flow cytometers that are currently supported by industry provide accurate CD4(+)-T-cell counts for monitoring human immunodeficiency virus disease but remain unaffordable for routine service work under resource-poor conditions. We therefore combined volumetric flow cytometry (measuring absolute lymph...
Antígenos CD4/análise, Linfócitos T CD4-Positivos/química, Citometria de Fluxo/métodos, d0174080, Antígenos Comuns de Leucócito, Linfócitos T CD4-Positivos/citologia, Síndrome da Imunodeficiência Adquirida/imunologia, Contagem de Células/métodos, Síndrome da Imunodeficiência Adquirida/diagnóstico, Antígenos Comuns de Leucócito/análise
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CD45-assisted PanLeucogating for accurate, cost-effective dual-platform CD4+ T-cell enumeration

Glencross, deborah; Scott, lesley, e; Jani, jlesh, v; Barnett, david; Janossy, george.
Cytometry, Part A; 50 (2), 2002
Background: North American and European guidelines for dual-platform (DP) flow cytometry recommendabsolute CD4 T-cell counts to be calculated from two parameters: the absolute lymphocyte counts obtainedon a hematology analyzer and the percentages of CD4 cells among lymphocytes (CD4%/lympho) obtainedby fl...
Linfócitos T CD4-Positivos/citologia, Citometria de Fluxo/economia, Citometria de Fluxo/métodos, Citometria de Fluxo/instrumentação, Imunofenotipagem/instrumentação, Imunofenotipagem/economia, Antígenos Comuns de Leucócito/biossíntese, Controle de Qualidade
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