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Genetic characterisation of south african and mozambican bovine rotaviruses reveals a typical bovine-like artiodactyl constellation derived through multiple reassortment events

Strydom, Amy; Donato, celeste m; Nyaga, martin m; Boene, simone s; Peenze, Ina; Mogotsi, Milton T; João, Eva D; Munlela, benilde; Potgieter, A. Christiaan; Seheri, mapaseka L; de Deus, Nilsa; O’Neill, hester G.
PloS pathog; 20 (2), 2022
This study presents whole genomes of seven bovine rotavirus strains from South Africa and Mozambique. Double-stranded RNA, extracted from stool samples without prior adaptation to cell culture, was used to synthesise cDNA using a self-annealing anchor primer ligated to dsRNA and random hexamers. The cDNA...
África do Sul, Rotavirus/genética, Infecções por Rotavirus/veterinária, Doenças dos Bovinos/virologia, Moçambique, Genótipo, Genoma Viral/genética
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Prevalence and genome characterization of porcine rotavirus A in southern Mozambique

Boane, Simone S; João, Eva D; Strydom, Amy; Munlela, Benilde; Chissaque, Assucênio; Bauhofer, Adilson Fernando Loforte; Nabetse, Elvino; Dalilo, Latifo; Cala, Aida; Mapaco, Lourenço; Chilaúle, Jorfélia; O'Neill, Hester G; Deus, nilza.
Infect. gent. evol; 87 (), 2021
Rotavirus A (RVA) is an important pathogen causing gastroenteritis in many species, including humans and pigs. The objective of this study was to determine the prevalence of RVA in pigs from smallholdings and commercial farms in southern Mozambique and characterize the complete genomes of selected strain...
Moçambique/epidemiologia, Rotavirus/isolamento & purificação, Rotavirus/genética, Suínos/virologia, Análise de Sequência de RNA, Fezes/virologia, Genótipo, Genoma Viral, Infecções por Rotavirus/epidemiologia, Filogenia
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Whole genome characterization and evolutionary analysis of g1p[8] rotavirus a strains during the pre- and post-vaccine periods in mozambique (2012–2017)

Munlela, Benilde; João, eva d; Donato, celeste m; Strydom, amy; Boene, simone s; Chissaque , assucênio; Bauhofer, adilson f. l; Langa, jerónimo; Cassocera, Marta; Cossa-Moiane, Idalécia; Chilaúle, jorfélia j; O’Neill, hester g; de Deus, nilsa.
PloS pathog; 9 (), 2020
Mozambique introduced the rotarix® vaccine (gsk biologicals, rixensart, belgium) into the na-tional immunization program in september 2015. Although g1p[8] was one of the most prevalent genotypes between 2012 and 2017 in mozambique, no complete genomes had been sequenced to date. here we report whole ge...
Genoma Viral/genética, Filogenia, Teorema de Bayes, Evolução Molecular, Vacinas contra Rotavirus, Sequenciamento Completo do Genoma, Rotavirus/genética, Moçambique
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