Resultados: 4

Affordable CD4(+)-T-cell counting by flow cytometry: CD45 gating for volumetric analysis

The flow cytometers that are currently supported by industry provide accurate CD4(+)-T-cell counts for monitoring human immunodeficiency virus disease but remain unaffordable for routine service work under resource-poor conditions. We therefore combined volumetric flow cytometry (measuring absolute lymph...

Helios + Regulatory T cell frequencies are correlated with control of viral replication and recovery of absolute CD4 T cells counts in early HIV-1 infection

BMC immunol. (Online); 18 (1), 2017
Background: The acute phase of HIV infection is characterized by massive depletion of CD4 T cells, high viral plasma levels and pronounced systemic immune activation. Regulatory T cells (Tregs) have the potential to control systemic immune activation but also to suppress antigen specific T and B cell res...

Precise CD4 T-cell counting using red diode laser excitation: for richer, for poorer

Cytometry, Part A; 50 (2), 2002
Background: Measuring CD4 T-cell counts at low cost is relevant in dealing with the human immunodeficiency virus (HIV) epidemic throughout the developing world. The recently introduced novel concepts in gating strategies and sample stabilization facilitate affordable immunophenotyping by flow cytometry. ...

CD45-assisted PanLeucogating for accurate, cost-effective dual-platform CD4+ T-cell enumeration

Cytometry, Part A; 50 (2), 2002
Background: North American and European guidelines for dual-platform (DP) flow cytometry recommendabsolute CD4 T-cell counts to be calculated from two parameters: the absolute lymphocyte counts obtainedon a hematology analyzer and the percentages of CD4 cells among lymphocytes (CD4%/lympho) obtainedby fl...