Results: 139

Malignant Catarrhal Fever in Brazilian cattle presenting with neurological syndrome

Braz. j. microbiol; 48 (2), 2017
Abstract Malignant Catarrhal Fever (MCF) was investigated in the central nervous system of cattle with neurological syndrome. Two-hundred-ninety samples were analyzed by histology, and molecular methods to detect ovine herpesvirus type 2 (OvHV-2) were optimized and validated. The qualitative polymerase c...

A duplex endpoint PCR assay for rapid detection and differentiation of leptospira strains

Abstract INTRODUCTION: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. METHODS: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species,...

Progressive disseminated histoplasmosis: a systematic review on the performance of non-culture-based diagnostic tests

Braz. j. infect. dis; 21 (1), 2017
Abstract The diagnosis of progressive disseminated histoplasmosis is often a challenge to clinicians, especially due to the low sensitivity and long turnaround time of the classic diagnostic methods. In recent years, studies involving a variety of non-culture-based diagnostic tests have been published in...

Genotipos circulantes del virus de la hepatitis C en Colombia

Biomédica (Bogotá); 37 (1), 2017
Resumen Introducción: La infección crónica por el virus de la hepatitis C es un problema de salud pública y se estima que hay más de 180 millones de personas infectadas en el mundo. En Colombia no se conoce la epidemiología de la infección ni los genotipos virales más frecuentes. Objetivo. Desc...

Development and application of KASP marker for high throughput detection of AhFAD2 mutation in peanut

Background: Cultivated peanut (Arachis hypogaea L.) is a major oilseed crop worldwide. Fatty acid composition of peanut oil may affect the flavor and shelf life of the resulting food products. Oleic acid and linoleic acid are the major fatty acids of peanut oil. The conversion from oleic acid to linoleic...

Estudio clínico genético en pacientes con complejo de esclerosis tuberosa

Rev. chil. pediatr; 88 (1), 2017
El complejo de esclerosis tuberosa (CET) es una enfermedad autosómica dominante multisistémica producida por mutaciones en los genes supresores de tumores TSC1 o TSC2. Objetivo: Caracterizar clínica y genéticamente pacientes pediátricos con diagnóstico de CET. Pacientes y Método: Estudio descripti...

Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis

Braz. j. med. biol. res; 50 (4), 2017
Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiat...

Análise crítica do protocolo de tratamento da ferida operatória complicada após esternotomia para cirurgia cardíaca

Rev. bras. cir. plást; 32 (2), 2017
Introdução: As complicações de ferida operatória após esternotomia para acessos cirúrgicos para procedimentos cardiovasculares variam desde pequenas deiscências até mediastinite e osteomielite do esterno. Mediastinite e osteomielite do esterno associam-se a alto risco, alta morbidade e altas tax...

Development of EPIC-PCR Markers for Lutjanus purpureus (Lutjanidae-Perciformes) and their Potential Applicability in Population Analyses

An. acad. bras. ciênc; 89 (3,supl), 2017
ABSTRACT In the present study, a novel set of eight EPIC primers were developed for Lutjanus purpureus and assayed in five other marine teleosts including three lutjanids, one scianid and one anablepid. Most of the genomic regions used in this study presented genetic diversity indexes equal or greater th...

Evaluation of the Western blotting method for the diagnosis of congenital toxoplasmosis

J. pediatr. (Rio J.); 92 (6), 2016
Abstract Objective: To evaluate the Western blotting method for the detection of IgG anti-Toxoplasma gondii (T. gondii) (IgG-WB) in the serum of children with suspected congenital toxoplasmosis. Methods: We accompanied 47 mothers with acquired toxoplasmosis in pregnancy and their children, between June...