Results: 354

Determinación de la mutación S315T del gen katG en aislados resistentes a Isoniacida de Mycobacterium tuberculosis mediante PCR-RFLP

Infectio; 22 (4), 2018
Objetivo: determinar la mutación S315T del gen katG en aislados de Mycobacterium tuberculosis resistentes a isoniacida mediante la técnica PCR-RFLP. Materiales y métodos: A partir de 68 aislados de Mycobacterium tuberculosis se realizó el análisis de polimorfismo en productos de amplificación de...

Influencia del medio de cultivo sobre la cinética de crecimiento y expresión de la proteína recombinante Rv2626c de Mycobacterium tuberculosis H37Rv expresada en Streptomyces lividans TK24
Influence of the culture medium on the kinetics of growth and expression of the recombinant protein Rv2626c of Mycobacterium tuberculosis H37Rv expressed in Streptomyces lividans TK24

Streptomyces lividans, ha ganado gran atención en los últimos tiempos, como vector de expresión y producción de proteínas de Mycobacterium tuberculosis de interés biomédico, como alternativa a su obtención tradicional en E. coli. La proteína Rv2626c, Proteína de Respuesta Hipóxica 1, es codifi...

Tuberculosis of the central nervous system in cattle in Paraíba, Brazil

Pesqui. vet. bras; 38 (11), 2018
This paper describes six cases of tuberculosis in the central nervous system (CNS) of cattle in the state of Paraíba in northeastern Brazil. We reviewed the autopsy reports of 851 bovine necropsies performed from 2003 to 2016. Seventy-three (8.6%) cattle were diagnosed with tuberculosis and six showed l...

Assessing and screening for T-cell epitopes from Mycobacterium tuberculosis RD2 proteins for the diagnosis of active tuberculosis

Braz. j. infect. dis; 22 (6), 2018
ABSTRACT The Region of D eletion 2 (RD2) of Mycobacterium tuberculosis encodes reserved antigens that contribute to bacterial virulence. Among these antigens, Rv1983, Rv1986, Rv1987, and Rv1989c have been shown to be immunodominant in infected cattle; however, their diagnostic utility has not been evalua...

Estandarizacion de la PCR en tiempo real por análisis de curvas melting para la detección de tuberculosis drogoresistente

Con-ciencia (La Paz); 6 (2), 2018
La tuberculosis es una enfermedad infecto-contagiosa, causada por diversas especies del Complejo Mycobacterium tuberculosis, actualmente se estima que un tercio de la población mundial se encuentra afectada por lo que representa una amenaza para la salud pública, principalmente por el surgimiento de ce...

Mycobacterium tuberculosis complex bacteremia among HIV and non-HIV patients in a Mexican tertiary care center

Braz. j. infect. dis; 22 (5), 2018
ABSTRACT Objectives: To determine the factors associated with Mycobacterium tuberculosis complex-positive blood culture. Methods: Case-control study. Sociodemographic, clinical and laboratory data were collected from 2000 to 2015. Results: We reviewed medical records of 533 patients with culture-prove...

Clinical impact and cost analysis of the use of either the Xpert MTB Rif test or sputum smear microscopy in the diagnosis of pulmonary tuberculosis in Rio de Janeiro, Brazil

Abstract INTRODUCTION: The molecular test Xpert MTB/RIF (Xpert) has been recommended for use in the diagnosis of pulmonary tuberculosis (PTB); however, data on the cost of incorporating it under routine conditions in high-burden countries are scarce. The clinical impact and costs incurred in adopting th...

Aspectos epidemiológicos dos pacientes notificados com tuberculose na microrregião de Umuarama – Noroeste Paranaense de 2009 a 2014

A tuberculose é uma doença infecciosa e transmissível, causada pelo bacilo aeróbico Mycobacterium tuberculosis ou por qualquer uma das sete espécies que integram este complexo, afeta prioritariamente os pulmões, embora possa acometer outros órgãos. A apresentação pulmonar é a mais frequente e ...

Diagnóstico molecular de tuberculosis multidrogorresistente en muestras de esputo mediante el análisis de curvas de melting

RESUMEN Objetivos. Analizar curvas de melting para el diagnóstico de tuberculosis multidrogorresistente a partir de muestras de esputo. Materiales y métodos. Se colectaron muestras de esputo (n = 250) de pacientes con sospecha clínica de tuberculosis pulmonar según resultado de baciloscopia y cul...

Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil

Braz. j. microbiol; 49 (3), 2018
Abstract DNA genotyping of Mycobacterium tuberculosis has been widely applied in the understanding of disease transmission in many countries. The purpose of this study was to genotype the strains of M. tuberculosis isolated in patients with new tuberculosis (TB) cases in Minas Gerais, as well as to compa...