Background: Invert sugar is used greatly in food and pharmaceutical industries. This paper describes scaling-up batch conditions for sucrose inversion catalyzed by the recombinant Pichia pastoris BfrA4X whole cells expressing Thermotoga maritima invertase entrapped in calcium alginate beads. For the firs...
Pichia/metabolismo,
Pichia/citología,
Hidrólisis,
Biotecnología/métodos,
Sacarosa/metabolismo,
Sacarosa/química,
Reactores Biológicos,
Biocatálisis,
Enzimas Inmovilizadas,
Cinética,
Alginatos,
Thermotoga maritima/enzimología
Abstract Purpose: To evaluate the inflammatory intensity and measure the tissue content of the proteins claudin-3 and occludin in the colonic mucosa without fecal stream submit to intervention with curcumin. Methods: Thirty-six rats were submitted to a proximal colostomy and a distal mucous fistula and...
Claudina-3/análisis,
Colon/química,
Curcuma/química,
Enema/métodos,
Mucosa Intestinal/química,
Ocludina/análisis,
Aceites de Plantas/farmacología,
Colon/efectos de los fármacos,
Colon/patología,
Colostomía,
Heces,
Inmunohistoquímica,
Mucosa Intestinal/efectos de los fármacos,
Mucosa Intestinal/patología,
Ratas Wistar
Abstract Purpose: To evaluate the expression of endothelial and inducible NOS in addition to the miRNA-27b in the corpus cavernosum and peripheral blood of healthy rats, diabetic rats, alcoholic rats and rats with both pathologies. Methods: Forty eight Wistar rats were divided into four groups: control...
Alcoholismo/metabolismo,
Diabetes Mellitus Experimental/metabolismo,
MicroARNs/análisis,
Óxido Nítrico Sintasa de Tipo III/análisis,
Óxido Nítrico Sintasa de Tipo II/análisis,
Pene/química,
Alcoholismo/complicaciones,
Alcoholismo/fisiopatología,
Diabetes Mellitus Experimental/complicaciones,
Diabetes Mellitus Experimental/fisiopatología,
Inmunohistoquímica,
Pene/fisiopatología,
Ratas Wistar
Background: A simple and efficient strategy for agarase immobilization was developed with carboxyl-functionalized magnetic nanoparticles (CMNPs) as support. The CMNPs and immobilized agarase (agarase-CMNPs) were characterized by transmission electron microscopy, dynamic light scattering, vibrating sample...
Glicósido Hidrolasas/metabolismo,
Enzimas Inmovilizadas,
Nanopartículas de Magnetita/química,
Dispersión Dinámica de Luz,
Termogravimetría,
Microscopía Electrónica de Transmisión,
Magnetometría,
Difracción de Rayos X,
Estabilidad de Enzimas,
Oligosacáridos/metabolismo,
Catálisis,
Glicósido Hidrolasas/química
Background: Biomineralization is a significant process performed by living organisms in which minerals are produced through the hardening of biological tissues. Herein, we focus on calcium carbonate precipitation, as part of biomineralization, to be used in applications for environmental protection, mate...
Background: Traditional methods of obtaining arsenic have disadvantages such as high cost and high energy consumption. Realgar is one of the most abundant arsenic sulphide minerals and usually treated as waste in industry. The aim of the present study was to screen an arsenic tolerant bacterium used for ...
Neste trabalho, avaliou-se o potencial do uso de oxidantes quirais em oxidações enantiosseletivas de compostos orgânicos de boro. É de conhecimento geral que compostos orgânicos de boro, especialmente ésteres e ácidos borônicos são facilmente oxidados por hidroperóxidos em meio básico. No enta...
Experimental tooth movement has been shown to induce inflammation and release of
chemical mediators. Inflammation can also alter nerve function that can be measured with
Quantitative Sensory Testing (QST). Various authors have studied orthodontic pain and the
different factors that modify it. But, to our...
Aparatos Ortodóncicos/efectos adversos,
Técnicas de Movimiento Dental/instrumentación,
Técnicas de Movimiento Dental/efectos adversos,
Líquido del Surco Gingival/química,
Citocinas/análisis,
Factor de Necrosis Tumoral alfa/análisis,
Dolor Facial/fisiopatología,
Dolor Facial/etiología,
Dimensión del Dolor,
Análisis de Varianza,
Ensayo de Inmunoadsorción Enzimática,
Estadísticas no Paramétricas
The focus of our studies has been the root substrate dentin, once root caries
prevalence has been reported as increasing in part due to people living longer and
maintaining their natural teeth into old age. Furthermore, this condition is highly
prevalent in patients undergoing head-and-neck radiotherapy ...
O propósito deste trabalho foi avaliar, in-vitro, a alteração de cor da cerâmica de dissilicato de lítio, após o envelhecimento artificial acelerado (EAA), variando-se os seguintes aspectos: processo de confecção (cerâmica monolítica e com infraestrutura coberta por cerâmica de revestim...
Cerámica/química,
Porcelana Dental/química,
Coloración de Prótesis,
Diseño de Prótesis Dental,
Cementos de Resina/química,
Espectrofotometría,
Color,
Ensayo de Materiales,
Análisis de Varianza,
Factores de Tiempo,
Colorimetría,
Aleaciones de Cerámica y Metal,
Reproducibilidad de los Resultados
