El ADN en la identificación humana: [Editorial]
Med. lab; 20 (9-10), 2014
Results: 424
Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker
Electron. j. biotechnol; 16 (6), 2013
Background: Genetic and epigenetic changes (DNA methylation) were examined in the tissue-culture propagated interspecific potato somatic hybrids between dihaploid Solanum tuberosum and S. pinnatisectum. Amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP) ...
Solanum tuberosum/genética, Variación Genética, Epigenómica, Técnicas In Vitro, Metilación de ADN, Polimorfismo Genético, Análisis del Polimorfismo de Longitud de Fragmentos Amplificados, Solanum tuberosum/crecimiento & desarrollo, ADN/aislamiento & purificación, Hibridación Genética, ADN de Plantas
Genetic diversity among native varieties and commercial cultivars of Solanum tuberosum ssp. tuberosum L. present in Chile
Electron. j. biotechnol; 16 (6), 2013
Background: The object of this work was to determine the genetic and allelic diversity of Solanum species present in Chile, assessing allelic distribution among native varieties and commercial cultivars of Solanum tuberosum ssp. tuberosum L., using microsatellite markers. Results: A high level of allelic...
Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana
Electron. j. biotechnol; 16 (6), 2013
Background: Cryopreservation refers to the storage of a living organism at ultra-low-temperature for long-term preservation of plant germplasm. The effect of cryopreservation on the efficiency of exogenous gene genetic transformation and expression level were studied herein. In this work, transgenic Arab...
Optimization of transgenesis conditions for the generation of CXCL2-luciferase reporter mice line
Electron. j. biotechnol; 16 (6), 2013
Background: Transgenesis by microinjection has been widely used for the generation of different mouse models. Different variables of the procedure may critically affect the efficiency of the process. A DNA construction that carries the CXCL2 promoter gene and firefly luciferase has been used to optimize ...
Utilidade da triagem dos anticorpos anti-dsDNA por quimioluminescência, seguida de confirmação por imunofluorescência indireta
Rev. bras. reumatol; 53 (5), 2013
OBJETIVO: Avaliar o desempenho de um imunoensaio quimioluminescente (CLIA) para os anticorpos anti-dsDNA, utilizando como referência o teste de imunofluorescência indireta (IFI) sobre Crithidia luciliae. MÉTODOS: O sistema de automação foi previamente aprovado com 81% de eficiência, 100% de sensibi...
Quantification of Leishmania infantum DNA in the bone marrow, lymph node and spleen of dogs
Rev. bras. parasitol. vet; 22 (3), 2013
The aim of the present study was to quantify the parasite load of Leishmania infantum in dogs using real-time PCR (qPCR). Bone marrow, lymph node and spleen samples were taken from 24 dogs serologically positive for L. infantum that had been put down by the official epidemiological surveillance service. ...
Nucleic acid recovery from thyroid fine-needle cytology slides
Arq. bras. endocrinol. metab; 57 (6), 2013
Perspectives in isolation of microRNA from thyroid fine-needle aspiration: reply to the letter "Nucleic acid recovery from thyroid fine-needle cytology slides"
Arq. bras. endocrinol. metab; 57 (5), 2013
Utilização de culturas padrões de salmonella para diagnóstico molecular
Arq. ciências saúde UNIPAR; 17 (1), 2013
Diagnóstico quanto à presença de microrganismos patogênicos nos alimentos é indispensável para garantir a qualidade e a segurança alimentar. Registros epidemiológicos oficiais apontam a Salmonella sp. como um dos principais microrganismos causadores de doenças de origem alimentar no estado do Pa...