LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud | LILACS

Results: 222

Comparison of different methods for total RNA extraction from sclerotia of Rhizoctonia solani

Electron. j. biotechnol; 17 (1), 2014
Background Rhizoctonia solani (teleomorph: Thanatephorus cucumeris) is one of the most important pathogens of rice (Oryza sativa L.) that causes severe yield losses in all rice-growing regions. Sclerotia, formed from the aggregation of hyphae, are important structures in the life cycles of R. solani and ...

Direcionamento de antígenos para células dendríticas in vivo: uma nova estratégia para o desenvolvimento de vacina na paracoccidioidomicose

A paracoccidioidomicose (PCM) é a micose sistêmica mais frequente no Brasil. Na última década, foi demonstrado que é possível enviar antígenos diretamente para as células dendríticas utilizando o anticorpo αDEC205 e na presença de um estímulo de maturação, o resultado é a indução de uma ...

Differential expression and localization of ADAM10 and ADAM17 during rat spermatogenesis suggest a role in germ cell differentiation

Biol. Res; 47 (), 2014
BACKGROUND: Extracellular metolloproteases have been implied in different process such as cell death, differentiation and migration. Membrane-bound metalloproteases of the ADAM family shed the extracellular domain of many cytokines and receptor controlling auto and para/juxtacrine cell signaling in diffe...

The changes of muscle FGF-6 expression at different time points after exercise-induced muscle damage

West Indian med. j; 62 (9), 2013
OBJECTIVE: To investigate the changes of fibroblast growth factor (FGF)-6 expression in the regeneration and repair process after exercise-induced muscle damage (EIMD) and the relationship with skeletal muscle regeneration and repair. METHODS: The expression of FGF-6 at different time points was examined...

Ethylene treatment improves diosgenin accumulation in in vitro cultures of Dioscorea zingiberensis via up-regulation of CAS and HMGR gene expression

Electron. j. biotechnol; 16 (5), 2013
Background: The perennial medicinal herb Dioscorea zingiberensis is a very important plant used for steroid drug manufacturing for its high level of diosgenin in rhizome. Although the stimulation of diosgenin accumulation by ethylene has been reported in a few of plant species, its regulation is not yet ...

Influence of initial pH of the growing medium on the activity, production and genes expression profiles of laccase of Pleurotus ostreatus in submerged fermentation

Electron. j. biotechnol; 16 (4), 2013
Background: Enzymatic activity and laccase isoenzymes number of Pleurotus ostreatus grown in different pH values of the growing medium in submerged fermentation and incubated in buffer solutions of different initial pH values were determined. The expression profiles of five laccase genes (Lacc1, Lacc4, L...

RIC-8B, um fator trocador de nucleotídeo guanina (GEF), é essencial para a embriogênese

RIC-8B é uma proteína que apresenta, in vitro, atividade de fator de troca de nucleotídeos guanina (GEF). No entanto, seu papel in vivo não é conhecido. Dados anteriores do nosso laboratório demonstraram que essa proteína interage especificamente com Gαolf, que é uma proteína G exclusiva do sis...

Tipificación molecular del virus dengue 3 durante el brote epidémico de dengue clásico en Lima, Perú, 2005
Molecular typing of dengue 3 virus during the classic dengue outbreak in Lima-Peru, 2005

Objetivos: Identificar mediante trascripción reversa-reacción en cadena de la polimerasa (RT-PCR) y sitios específicos de restricción - reacción en cadena de la polimerasa (RSS-PCR) al agente causal del brote epidémico presentado en el distrito de Comas, Lima en abril del año 2005. Materiales y mÃ...

Relevance of micrometastases detected by reverse transcriptase-polymerase chain reaction for melanoma recurrence: systematic review and meta-analysis

Säo Paulo med. j; 121 (1), 2003
CONTEXT: Cutaneous melanoma presents significant morbidity and mortality. Nowadays, about 90 percent of them are diagnosed by clinical examination and most are localized melanomas. Sentinel node biopsy has brought about a new and interesting approach towards localized cutaneous melanoma. The meaning of m...

Construção e caracterização de mini-bibliotecas de EST geradas com RT-PCR de baixa estringencia e clonagem de uma apirase de Schistosoma mansoni

Este trabalho demonstrou a construção de minibibliotecas de "Expressed Sequence Tags" (EST) com o uso de RT-PCR de baixa estringencia e "primer" consenso-degenerado. Através do estudo de parâmetros críticos como concentração de sais, temperatura e velocidade de ciclagem, composição dos "primers"...