Los métodos fenotípicos empleados para la identificación de microorganismos dependen de procesos metabólicos que requieren de tiempos de incubación mínimos para alcanzar resultados confiables. La espectrometría de masas MALDI-TOF (desorción/ionización láser asistida por una matriz con detecció...
Abstract INTRODUCTION. This study aimed to evaluate different methods for differentiation of species of coagulase-negative staphylococci (CoNS) that caused infections in hospitalized immunocompromised patients. METHODS. A total of 134 CoNS strains were characterized using four different methods. RES...
ABSTRACT Herein we report the case of a 10-year-old boy with an autosomal mosaic mutation who developed bacteremia. The causative agent was identified as Moraxella osloensis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA gene sequencing. In the pediatric popu...
Abstract In the previous study, we used genome shuffling to improve fengycin production of the original strain Bacillus amyloliquefaciens ES-2-4. After two rounds of genome shuffling, a high-yield recombinant FMB72 strain that exhibited 8.30-fold increase in fengycin production was obtained. In this stud...
Bacillus amyloliquefaciens/química,
Bacillus amyloliquefaciens/genética,
Bacillus amyloliquefaciens/metabolismo,
Proteínas Bacterianas/química,
Proteínas Bacterianas/genética,
Proteínas Bacterianas/metabolismo,
Barajamiento de ADN,
Genoma Bacteriano,
Lipopéptidos/biosíntesis,
Proteómica,
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
Os Staphylococcus coagulase-negativos (SCN) são encontrados na pele e mucosas de seres humanos e outros animais, já que algumas espécies são parte constituinte da microbiota normal destes mesmos sítios, e podem constituir um reservatório para SCN. Staphylococcus haemolyticus é a segunda espécie, ...
Staphylococcus haemolyticus/patogenicidad,
Biopelículas/efectos de los fármacos,
Farmacorresistencia Bacteriana Múltiple/genética,
Caenorhabditis elegans/microbiología,
Infección Hospitalaria/prevención & control,
Pruebas de Sensibilidad Microbiana/métodos,
Genes Bacterianos,
Proteínas Bacterianas/genética,
Infecciones Estafilocócicas/prevención & control,
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos,
Factores de Virulencia/análisis,
Resistencia a la Vancomicina,
Cultivo de Sangre/métodos,
Adhesión Bacteriana/inmunología,
Bacteriemia/microbiología
O objetivo deste trabalho foi introduzir a técnica de espectrometria de massa com fonte de ionização e dessorção a laser assistida por matriz e analisador de tempo-de-voo (MALDI-TOF) para incrementar o método tradicional microbiológico na detecção de Salmonella spp. e Escherichia coli em carcaç...
Abstract: Background: A key process in cell regulation is protein phosphorylation, which is catalyzed by protein kinases and phosphatases. However, phosphoproteomics studies are difficult because of the complexity of protein phosphorylation and the number of phosphorylation sites. Methods: We describ...
ABSTRACT Bloodstream infections (BSIs) are among the most concerning bacterial infections. They are one of the leading causes of morbidity and mortality, and occur in 30-70% of critical care patients. The prompt identification of the causative microorganism can help choosing the appropriate antimicrobial...
Abstract Mastitis adversely affects milk production and in general cows do not regain their full production levels post recovery, leading to considerable economic losses. Moreover the percentage decrease in milk production depends on the specific pathogen that caused the infection and enterobacteria are ...
Enterobacteriaceae/clasificación,
Enterobacteriaceae/metabolismo,
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción,
Bovinos,
Girasa de ADN/genética,
Enterobacteriaceae/aislamiento & purificación,
Genes Bacterianos,
Mastitis Bovina/diagnóstico,
Leche/microbiología,
Fenotipo,
Proteómica/métodos,
Análisis de Secuencia de ADN,
Mastitis Bovina/microbiología
Among its innumerous applications in Bacteriology, the Matrix-Assisted Laser Desorption Ionization-Time of
Flight Mass Spectrometry (MALDI-TOF MS) technique is evolving as a powerful tool for bacterial identification
and antimicrobial resistance investigation. Publications have evaluated the MALDI-TOF MS...
