Results: 47

Vascular endothelial growth factor gene variations as a risk predictor in disc degeneration

Einstein (Säo Paulo); 15 (4), 2017
ABSTRACT Objective: To evaluate the frequency of polymorphisms in the vascular endothelial growth factor (VEGF) gene, as well as to identify a potential risk haplotype among the polymorphic regions in this gene in patients with disc degeneration and in the Control Group. Methods: This study analyzed a ...

Development of a propidium monoazide-polymerase chain reaction assay for detection of viable Lactobacillus brevis in beer

Braz. j. microbiol; 48 (4), 2017
ABSTRACT The spoilage of beer by bacteria is of great concern to the brewer as this can lead to turbidity and abnormal flavors. The polymerase chain reaction (PCR) method for detection of beer-spoilage bacteria is highly specific and provides results much faster than traditional microbiology techniques. ...

Selection of suitable reference genes for abiotic stress-responsive gene expression studies in peanut by real-time quantitative PCR

Background: Because of its strong specificity and high accuracy, real-time quantitative PCR (RT-qPCR) has been a widely used method to study the expression of genes responsive to stress. It is crucial to have a suitable set of reference genes to normalize target gene expression in peanut under different ...

Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei, Mytilidae) in environmental samples

An. acad. bras. ciênc; 89 (2), 2017
ABSTRACT The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the a...

Real-time PCR assay for the diagnosis of pleural tuberculosis

Colomb. med; 48 (2), 2017
Abstract Introduction: The diagnosis of pleural tuberculosis requires an invasive and time-consuming reference method. Polymerase chain reaction (PCR) is rapid, but validation in pleural tuberculosis is still weak. Objective: To establish the operating characteristics of real-time polymerase chain re...

Detection of herpes simplex-1 and -2 and varicella zoster virus by quantitative real-time polymerase chain reaction in corneas from patients with bacterial keratitis

Arq. bras. oftalmol; 80 (2), 2017
ABSTRACT Objective: Bacterial keratitis occurs worldwide, and despite recent developments, it remains a potentially blinding condition. This study assesses the presence of herpes simplex virus (HSV-1 and -2) and varicella zoster virus (VZV) by quantitative real-time polymerase chain reaction (qPCR) in c...

Papel del factor neurotrófico de origen cerebral y de la neurotropina-3 en la relación de Chlamydophila pneumoniae con la esquizofrenia
The relationship of Chlamydophila pneumoniae with schizophrenia: The role of brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) in this relationship

Rev. argent. microbiol; 49 (1), 2017
Several pathogens have been suspected of playing a role in the pathogenesis of schizophrenia. Chronic inflammation has been proposed to occur as a result of persistent infection caused by Chlamydophila pneumoniae cells that reside in brain endothelial cells for many years. It was recently hypothesized th...

qPCR detection of Mycobacterium leprae in biopsies and slit skin smear of different leprosy clinical forms

Braz. j. infect. dis; 21 (1), 2017
Abstract Leprosy, whose etiological agent is Mycobacterium leprae, is a chronic infectious disease that mainly affects the skin and peripheral nervous system. The diagnosis of leprosy is based on clinical evaluation, whereas histopathological analysis and bacilloscopy are complementary diagnostic tools. ...

Expressão dos genes ALS3, HWP1, BCR1, TEC1, CPH1 e EFG1 de Candida albicans em biofilmes após inativação fotodinâmica

Os micro-organismos estão se tornando cada vez mais resistentes aos antimicrobianos e cepas de Candida albicans resistentes aos antifúngicos tem sido isoladas, assim, torna-se importante e necessário a realização de pesquisas que avaliem os efeitos de novos métodos terapêuticos, como a inativaçã...

Expression of heparanase in basal cell carcinoma and squamous cell carcinoma

An. bras. dermatol; 91 (5), 2016
Abstract: Background: Heparanase is an enzyme that cleaves heparan sulfate chains. Oligosaccharides generated by heparanase induce tumor progression. Basal cell carcinoma and squamous cell carcinoma comprise types of nonmelanoma skin cancer. Objectives: Evaluate the glycosaminoglycans profile and expre...