Kanegae, Marilia Pyles Patto;
Barreiros, Lucila Akune;
Mazzucchelli, Juliana Themudo Lessa;
Hadachi, Sonia Marchezi;
Guilhoto, Laura Maria de Figueiredo Ferreira;
Acquesta, Ana Lúcia;
Genov, Isabel Rugue;
Holanda, Silvia Maia;
Di Gesu, Regina Sumiko Watanabe;
Goulart, Ana Lucia;
Santos, Amélia Miyashiro Nunes dos;
Bellesi, Newton;
Costa-Carvalho, Beatriz Tavares;
Condino-Neto, Antonio.
Abstract Objective To apply, in Brazil, the T-cell receptor excision circles (TRECs) quantification technique using real-time polymerase chain reaction in newborn screening for severe combined immunodeficiency and assess the feasibility of implementing it on a large scale in Brazil. Methods 8715 newbor...
Factores de Edad,
Brasil,
Pruebas con Sangre Seca,
Tamizaje Neonatal/métodos,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Receptores de Antígenos de Linfocitos T/sangre,
Receptores de Antígenos de Linfocitos T/genética,
Valores de Referencia,
Reproducibilidad de los Resultados,
Sensibilidad y Especificidad,
Inmunodeficiencia Combinada Grave/sangre,
Inmunodeficiencia Combinada Grave/diagnóstico,
Estadísticas no Paramétricas,
Factores de Tiempo
Introduction: Human parechovirus (HPeV) belongs to the Picornaviridae family and has been described in viral meningoencephalitis and sepsis like illness in infants. Until now, 16 genotypes have been recognized, the most common are HPEV 1, 2 and 3; type 3 is most severe. Aims: To estimate the frequency of...
ABSTRACT Increased matrix metalloproteinases (MMPs) activity is a hallmark of periapical granulomas. However, the factors underlying the MMPs expression modulation in healthy and diseased periapical tissues remains to be determined. Objective In this study, we evaluated the association between the MMP1...
Estudios de Casos y Controles,
Citocinas/análisis,
Citocinas/genética,
Estudios de Asociación Genética,
Marcadores Genéticos,
Genotipo,
Metaloproteinasa 1 de la Matriz/análisis,
Metaloproteinasa 1 de la Matriz/genética,
Enfermedades Periapicales/genética,
Granuloma Periapical/genética,
Polimorfismo Genético,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Valores de Referencia,
Análisis de Regresión,
Factores de Riesgo,
Estadísticas no Paramétricas,
Regulación hacia Arriba
ABSTRACT Aging negatively affects bone/titanium implant interactions. Our hypothesis is that the unbalance between osteogenesis and adipogenesis induced by aging may be involved in this phenomenon. Objective We investigated the osteoblast and adipocyte differentiation of mesenchymal stem cells (MSCs) f...
Adipogénesis/fisiología,
Factores de Edad,
Envejecimiento/fisiología,
Fosfatasa Alcalina/análisis,
Proliferación Celular/fisiología,
Células Cultivadas,
Implantes Dentales,
Expresión Génica,
Lípidos/análisis,
Células Madre Mesenquimatosas/fisiología,
Osteoblastos/fisiología,
Osteogénesis/fisiología,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Propiedades de Superficie,
Titanio/química
ABSTRACT Objective The objective of this study was to clarify differences regarding HPV16 infection and gene amplification between the oral cavity and oropharynx in healthy individuals. Material and Methods The subjects were 94 healthy asymptomatic individuals (41 males, 53 females; mean age 58.6 yea...
Factores de Edad,
Recuento de Células,
Variaciones en el Número de Copia de ADN,
ADN Viral,
Amplificación de Genes/fisiología,
Papillomavirus Humano 16/genética,
Japón/epidemiología,
Boca/virología,
Orofaringe/virología,
Infecciones por Papillomavirus/epidemiología,
Infecciones por Papillomavirus/virología,
Prevalencia,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Factores de Riesgo,
Factores de Tiempo
Enteric disease is a multifactorial problem in chickens, which causes gastrointestinal alterations, elevated feed conversions and impairment. In the last years, several enteric viruses were implicated in enteric disease; case reports have shown their presence alone or in concomitant infections during out...
Síndromes de Malabsorción/diagnóstico,
Síndromes de Malabsorción/veterinaria,
Avastrovirus/aislamiento & purificación,
Parvovirus/aislamiento & purificación,
Pollos/virología,
Páncreas/fisiopatología,
Bazo/virología,
Enfermedades Gastrointestinales/veterinaria,
Raquitismo/diagnóstico,
Raquitismo/veterinaria,
Enanismo/diagnóstico,
Enanismo/veterinaria,
Reacción en Cadena en Tiempo Real de la Polimerasa/veterinaria
Background: Agave tequilana has a great economic importance in Mexico in order to produce alcoholic beverages and bioenergy. However, in this species the structure and organization of the rDNAs in the genome are limited, and it represents an obstacle both in their genetic research and improvement as well...
Contexto: Um dos requisitos para garantir a instituição de um tratamento adequado aos pacientes acometidos por leucemia/linfoma associada ao HTLV-1 é a confirmação diagnóstica da infecção por HTLV-1. Essa confirmação, dá-se hoje por meio de exames laboratoriais, a saber: ELISA, WESTERN BLOT e ...
Este estudo verificou o desempenho de três técnicas de PCR quantitativa (Real-Time) para o diagnóstico de Peste Suína Africana, uma doença exótica no Brasil, a partir de amostras de tecidos. As três técnicas escolhidas baseiam-se na amplificação de sequências do gene da proteína viral VP72 e ...
This present study was developed with the objective of detect Salmonella sp. by conventional bacteriology and qPCR techniques in samples of flooring material from transport crates (meconium); raising environment (swab of cages and drinking fountains); cloacal swab; food and insects from growing, rearing ...
