Results: 27

Producción de plásmidos recombinantes para su uso como controles de la técnica RT-PCR para el diagnósticode los virus Chikungunya y Zika

El diagnóstico molecular de arbovirus es indispensable para identificar agentes etiológicos, particularmente en zonas endémicas para al menos uno de ellos. Estas deben ser validadas con controles positivos, los cuales están clásicamente representados por virus vivos, cuya obtención puede ser riesgo...

Biopolymer Xanthan: A New Adjuvant for DNA Vaccines

Abstract DNA vaccines have been evaluated as an option to prevent several diseases. In this study, the capacity of the xanthan biopolymer to improve the DNA vaccines immune response, administered intramuscularly, was evaluated. The experimental vaccines consisted of genes encoding fragments of the protei...

Método rápido de verificación de mutantes de Escherichia coli empleados en ingeniería genética
Rapid verification method of Escherichia coli mutants used in genetic engineering

Para las metodologías de ADN recombinante, los biólogos moleculares emplean mutantes de Escherichia coli que son adquiridos de kit comerciales o de colecciones microbianas especializadas. En la práctica estos mutantes son conservados por pases sucesivos o en un banco poco caracterizado. No seguir el s...

New molecular biologist perspective and insight: DNA topoisomerases production by recombinant DNA technology for medical laboratory application and pharmaceutical industry

Electron. j. biotechnol; 16 (6), 2013
DNA topoisomerases are essential enzymes that control the topological state of DNA replication during mitosis. These enzymes are classified based on their mechanisms and physical properties. During mitosis, superhelical DNA must be unwound or relaxed by DNA topoisomerases prior to a decoding step by DNA ...

DNA removal from a purification process of recombinant hepatitis B surface antigen

Electron. j. biotechnol; 10 (1), 2007
We studied the capacity of an API-rHBsAg purification process to eliminate DNA contamination from yeast-host cell. Firstly, was demonstrated consistency of manufacturing purification process to remove DNA, from (3.9 + - 1.9)108 pg/dose in starting material to (3.4 + - 1.6) pg/dose, equivalent to 8.2 log ...

Fermentaçäo alcoólica de material amiláceo por levedura amilolítica recombinante em reator com células imobilizadas

O objetivo geral deste trabalho foi o estudo do desempenho da levedura recombinante Saccharomyces cerevisiae L36, que expressa os genes da ALPHA-amilase de Bacillus subtilis, glicoamilase de Aspergillus awamori e maltase de Saccharomyces cerevisiae, imobilizada em gel de pectina, no processo de fermenta�...