Results: 80

Cross-genera amplification and identification of Colpodella sp. with Cryptosporidium primers in fecal samples of zoo felids from northeast China

Braz. j. biol; 83 (), 2023
Abstract The protozoans include many intracellular human pathogens. Accurate detection of these pathogens is necessary to treat the diseases. In clinical epidemiology, molecular identification of protozoan is considered a more reliable and rapid method for identification than microscopy. Among these prot...

Evaluación de pruebas inmunológicas en el diagnóstico de Giardia duodenalis y Cryptosporidium spp, Honduras
Evaluation of immunologic tests in the diagnosis of Giardia duodenalis and Cryptosporidium spp., Honduras

Rev. méd. hondur; 90 (1), 2022
Antecedentes: No conocemos datos sobre evaluación de pruebas inmunológicas para mejorar el diagnóstico de Giardia duodenalis y Cryptosporidium spp., agentes etiológicos de diarrea de importancia mundial, en Honduras. Objetivos: Comparar dos pruebas inmunológicas para el diagnóstico de Giardia y Cry...

Use of laboratory methodologies to confirm an outbreak of acute diarrheal disease caused by multiple pathogens

The aim of this study was to investigate an outbreak caused by protozoa, which occurred in a municipality in the Brazil southern region. The investigations were carried out analyzing 47 fresh stool samples and 26 water samples by parasitological and molecular methods, as well as...

Validation of a one-tube nested real-time PCR assay for the detection of Cryptosporidium spp. in avian fecal samples

Abstract The aim of this study was to validate a one-tube nested real-time PCR assay followed by genetic sequencing to detect and identify Cryptosporidium species and genotypes in birds. A total of 443 genomic DNA extracted from avian fecal samples were analyzed by one-tube nested real-time PCR and conve...

Caracterización físico-química y microbiológica del agua de un municipio del departamento de Boyacá (Colombia)

Introducción: El agua salubre y fácilmente accesible es importante para la salud pública, si se utiliza para beber, uso doméstico, producir alimentos o fines recreativos. El agua contaminada y el saneamiento deficiente están re-lacionados con la transmisión de enfermedades. Objetivo: Evaluar las ...

Standardization of molecular techniques for the detection and characterization of intestinal protozoa and other pathogens in humans

Background: The intrinsic sensitivity limitations of basic parasitological methods, along with the particular biological characteristics of parasites, make these methods ineffective to differentiate morphologically indistinguishable species. Molecular detection and characterization techniques could be u...

Prevalence of cestodes infection among school children of urban parts of Lower Dir district, Pakistan

Braz. j. biol; 82 (), 2022
Tapeworms of zoonotic importance have been described as a leading public health problem. Current research was aim to assess the prevalence of tapeworms among 5-12years school children residing in district Lower Dir, Pakistan from January 2019-December 2019. The wet mount preparation in saline/iodine/meth...

Prevalence of infection by Cryptosporidium spp. in calves and associated risk factors in Northeastern Brazil

Cryptosporidium spp. are zoonotic protozoa, frequently associated with diarrhea in calves, which are responsible for important economic losses. The aim of this study was to assess the prevalence of infection by Cryptosporidium spp. and its associated risk factors among calves raised in a milk production ...

Detección molecular de patógenos entéricos en niños con diarrea en un hospital centinela de vigilancia de rotavirus en Chile

Rev. chil. infectol; 38 (1), 2021
INTRODUCCIÓN: Las diarreas de causa infecciosa son un problema de salud pública, especialmente en niños bajo los cinco años. La identificación de los agentes etiológicos puede ser relevante para el manejo del cuadro clínico y, desde el punto de vista epidemiológico, para la implementación de med...

Caracterização molecular de cistos de Giardia spp. e de oocistos de Cryptosporidium spp. recuperados de amostras de água de reúso

O aumento da demanda de recursos hídricos causado pelo crescimento da população mundial, pela necessidade de produção de alimentos e utilização na indústria é uma realidade. Como enfrentamento a este cenário de segurança hídrica, existe uma crescente tendência mundial de uso de águas recicl...