BACKGROUND: Molecular brain therapies require the development of molecular switches to control gene expression in a limited and regulated manner in time and space. Light-switchable gene systems allow precise control of gene expression with an enhanced spatio-temporal resolution compared to chemical induc...
Lima, C. C. V;
Ayres, M. C. C;
Pinheiro, R. R;
Costa, J. N;
Andrioli, A;
Souza, T. S;
Azevedo, D. A. A;
Santos, V. W. S;
Araújo, J. F;
Sousa, A. L. M;
Peixoto, R. M;
Damasceno, E. M;
Costa Neto, A. O.
With the objective of detecting the presence of caprine lentivirus (CLV) in ewe milk and in ram semen, ten matrixes and four reproducers experimentally infected with CLV were used. Samples of ewe milk were collected during the four months of lactation, five collections per animal, totaling 50 samples. Re...
Abstract Small ruminant lentiviruses isolated from peripheral blood leukocytes and target organs can be propagated in vitro in fibroblasts derived from goat synovial membrane cells. These cells are obtained from tissues collected from embryos or fetuses and are necessary for the establishment of the fibr...
Lentivirus/fisiología,
Células Madre Mesenquimatosas/virología,
Adipogénesis,
Biomarcadores,
Técnicas de Cultivo de Célula,
Diferenciación Celular,
Células Cultivadas,
Condrogénesis,
Efecto Citopatogénico Viral,
Cabras,
Inmunofenotipificación,
Técnicas In Vitro,
Células Madre Mesenquimatosas/citología,
Células Madre Mesenquimatosas/metabolismo,
Células Madre Mesenquimatosas/patología,
Osteogénesis,
Replicación Viral,
Cordón Umbilical/citología
BACKGROUND: Ubiquitin specific peptidase 39 (USP39), an essential factor in the assembly of the mature spliceosome complex, has an aberrant expression in several cancer. However, its function and the corresponding mechanism on human osteosarcoma has not been fully explored yet. METHODS: The mRNA and DNA ...
Apoptosis,
Técnicas de Silenciamiento del Gen/métodos,
Osteosarcoma/enzimología,
Osteosarcoma/patología,
Proteasas Ubiquitina-Específicas/metabolismo,
Proteasas Ubiquitina-Específicas/genética,
Línea Celular Tumoral,
Proliferación Celular,
Citometría de Flujo,
Regulación Neoplásica de la Expresión Génica,
Vectores Genéticos,
Lentivirus,
Ensayo de Tumor de Célula Madre
BACKGROUND: Zinc finger RNA binding protein (ZFR) is involved in the regulation of growth and cancer development. However, little is known about ZFR function in pancreatic cancer. METHODS: Herein, to investigate whether ZFR is involved in tumor growth, Oncomine microarray data was firstly used to evaluat...
Técnicas de Silenciamiento del GenMétodos,
Neoplasias Pancreáticas/patología,
ARN Interferente Pequeño/farmacología,
Proteínas de Unión al ARN/metabolismo,
Western Blotting,
Línea Celular Tumoral,
Proliferación Celular/genética,
Supervivencia Celular,
Células Cultivadas,
Citometría de FlujoMétodos,
Formazáns,
Lentivirus/genética,
Terapia Molecular Dirigida,
Invasividad Neoplásica/genética,
Invasividad Neoplásica/patología,
Neoplasias Pancreáticas/genética,
Neoplasias Pancreáticas/metabolismo,
Proteínas de Unión al ARN/genética,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Sales de Tetrazolio
Pan, Zeya;
Pan, Hao;
Zhang, Jin;
Yang, Yun;
Liu, Hui;
Yang, Yuan;
Huang, Gang;
Ni, Junsheng;
Huang, Jian;
Zhou, Weiping.
BACKGROUND: Ubiquitin Specific Peptidase 39 (USP39) is a 65 kDa SR-related protein involved in RNA splicing. Previous studies showed that USP39 is related with tumorigenesis of human breast cancer cells. RESULTS: In the present study, we investigated the functions of USP39 in human hepatocellular carcino...
Carcinoma Hepatocelular/patología,
Ciclo Celular,
Proliferación Celular,
Lentivirus/genética,
Neoplasias Hepáticas/patología,
Proteínas de Neoplasias/metabolismo,
Interferencia de ARN/fisiología,
Proteasas Ubiquitina-Específicas/metabolismo,
Apoptosis,
Western Blotting,
Carcinoma Hepatocelular/enzimología,
Ciclo Celular/genética,
Línea Celular Tumoral,
Proliferación Celular/genética,
Regulación Neoplásica de la Expresión Génica/genética,
Técnicas de Silenciamiento del Gen,
Silenciador del Gen,
Técnicas de Transferencia de Gen,
Técnicas In Vitro,
Neoplasias Hepáticas/enzimología,
Proteínas de Neoplasias/genética,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Proteasas Ubiquitina-Específicas/genética
Este trabalho teve como objetivo comparar os resultados do diagnóstico do Lentivírus Caprino, por Imunodifusão em Gel de ágar - IDGA, utilizando o kit comercial americano e o kit nacional produzido com cepa CAEV Cork. Foram utilizados dois rebanhos, sendo um da Embrapa Caprinos submetido a doze anos ...
Os lentivírus de pequenos ruminantes (SRLV), cujos protótipos säo os vírus da Artrite-Encefalite Caprina (CAEV) e Maedi-Visna, säo patógenos amplamente distribuidos, os quais causam doenças degenerativas progressivas lentas em caprinos e ovinos, determinando importantes perdas econômicas. Estes v...
