Abstract The development of stable cell lines producing recombinant proteins is very time-consuming and laborious. One of the practical approaches successfully performed is Fluorescence-Activated Cell Sorting (FACS). A mutated chimeric tissue plasminogen activator (mt-PA) was developed by removing the fi...
BACKGROUND:
Maize is one of the most important crops worldwide and has been a target of nuclear-based transformation biotechnology to improve it and satisfy the food demand of the ever-growing global population. However, the maize plastid transformation has not been accomplished due to the recalcitrant c...
Zea mays/genética,
Cloroplastos/genética,
Cloroplastos/metabolismo,
Proteínas Fluorescentes Verdes/metabolismo,
Nicotiana/metabolismo,
Proteínas Fluorescentes Verdes/genética,
Plastidios/genética,
Plantas Modificadas Genéticamente,
Biotecnología,
Transformación Genética,
Reacción en Cadena de la Polimerasa,
Escherichia coli,
Genoma del Cloroplasto
BACKGROUND: Duchenne muscular dystrophy (DMD) is a devastating genetic muscular disorder with no effective treatment that is caused by the loss of dystrophin. Human induced pluripotent stem cells (hiPSCs) offer a promising unlimited resource for cell-based therapies of muscular dystrophy. However, their ...
BACKGROUND Bacillus Calmette-Guérin (BCG) is considered a promising live bacterial delivery system. However, several proposals for rBCG vaccines have not progressed, mainly due to the limitations of the available expression systems. OBJECTIVES To obtain a set of mycobacterial vectors using a range of...
Abstract Objective Ameloblastoma is a representative odontogenic tumor comprising several characteristic invasive forms, and its pathophysiology has not been sufficiently elucidated. A stable animal experimental model using immortalized cell lines is crucial to explain the factors causing differences a...
Ameloblastoma/patología,
Línea Celular Tumoral,
Células Cultivadas,
Colágeno,
Modelos Animales de Enfermedad,
Combinación de Medicamentos,
Proteínas Fluorescentes Verdes/análisis,
Inmunohistoquímica,
Laminina,
Neoplasias Experimentales/patología,
Proteoglicanos,
Reproducibilidad de los Resultados,
Factores de Tiempo
The enrichment of therapeutic protein production yield in mammalian cell cultures by modulating mRNA stability is a fairly new strategy in biotechnological applications. Here, we describe the application of 3′-untranslated region (3′UTR) from RNA viral genome to modulate mRNA stability.The data obtai...
Background: To examine the usefulness of green fluorescent protein (GFP) mice for studying the interactions between normal cells and tumor cells in a host, we used a melanoma model in such “green” mice [C57BL/6-Tg (CAG-EGFP)1Osb mice]. Mice were given a subcutaneous injection of B16-F10 cells, and th...
Background: Capsicum is a genus of an important spice crop that belongs to the chili lineage. However, many Capsicum species (family Solanaceae) are known to be recalcitrant to genetic transformation and in vitro regeneration, thus hampering the effort in using Capsicum species for detailed biological in...
Abstract INTRODUCTION: Pseudo-infectious yellow fever viral particles (YFV-PIVs) have been used to study vaccines and viral packaging. Here, we report the development of a packaging cell line, which expresses the YFV prM/E proteins. METHODS: HEK293 cells were transfected with YFV prM/E and C (84 nt) ...
Citometría de Flujo,
Técnica del Anticuerpo Fluorescente Indirecta,
Proteínas Fluorescentes Verdes,
Células HEK293,
Vacunas de Partículas Similares a Virus/genética,
Vacunas de Partículas Similares a Virus/inmunología,
Ensamble de Virus/genética,
Ensamble de Virus/inmunología,
Replicación Viral/genética,
Replicación Viral/inmunología,
Virus de la Fiebre Amarilla/genética,
Virus de la Fiebre Amarilla/inmunología
Abstract Although infections with NonTuberculous Mycobacteria have become less common in AIDS patients, they are important opportunistic infections after surgical procedures, likely because they are ubiquitous and not efficiently killed by many commonly used disinfectants. In Venezuela there have recentl...
Antibacterianos/farmacología,
Desinfectantes/farmacología,
Expresión Génica,
Proteínas Fluorescentes Verdes,
Micobacterias no Tuberculosas/efectos de los fármacos,
Compuestos de Amonio Cuaternario/farmacología,
Relación Dosis-Respuesta a Droga,
Proteínas Fluorescentes Verdes/genética,
Pruebas de Sensibilidad Microbiana,
Micobacterias no Tuberculosas/clasificación,
Micobacterias no Tuberculosas/genética,
Plásmidos/genética,
Proteínas Recombinantes de Fusión/genética
