Introducción: El sistema inmunológico puede reconocer una gran cantidad de antígenos cuando está expuesto a ellos. Los linfocitos B producen gran variedad de anticuerpos, con el fin de generar la especificidad de los receptores para el reconocimiento de dichos antígenos. La presencia de anticuerpos ...
Background: The development of a potential single culture that can co-produce hydrogen and ethanol is beneficial for industrial application. Strain improvement via molecular approach was proposed on hydrogen and ethanol co-producing bacterium, Escherichia coli SS1. Thus, the effect of additional copy of ...
Escherichia coli/metabolismo,
Escherichia coli/enzimología,
Hidrógeno/metabolismo,
Etanol/metabolismo,
Fermentación,
Glucosa/metabolismo,
Medios de Cultivo,
Hidrogenasas/metabolismo,
Hidrogenasas/genética,
Alcalinización,
Glicerol/metabolismo,
Biodegradación Ambiental,
Recombinación Genética
Background: Catalase (CAT) is an important enzyme that degrades H2O2 into H2O and O2. To obtain an efficient catalase, in this study, a new strain of high catalase-producing Serratia marcescens, named FZSF01, was screened and its catalase was purified and characterized. Results: After optimization of fer...
Serratia marcescens/enzimología,
Serratia marcescens/genética,
Catalasa/metabolismo,
Catalasa/aislamiento & purificación,
Catalasa/genética,
Fermentación,
Peróxido de Hidrógeno/metabolismo,
ARN Ribosómico 16S,
Análisis de Secuencia de ADN,
Electroforesis,
Cromatografía Liquida,
Escherichia coli/genética,
Escherichia coli/metabolismo,
Recombinación Genética,
Cinética
Background: Zymomonas mobilis is a Gram-negative microaerophilic bacterium with excellent ethanol-producing capabilities. The RecET recombination system provides an efficient tool for direct targeting of genes in the bacterial chromosome by PCR fragments. Results: The plasmids pSUZM2a-RecET and pSUZM2a-R...
Background: MicroRNAs (miRNAs) are endogenous noncoding RNAs that regulate various biological processes. miR-125b is a miRNA that has been reported to be critical for hair follicle (HF) morphogenesis and development. We identified that the expression of miR-125b varies during an individual hair cycle (an...
Folículo Piloso/crecimiento & desarrollo,
Cabras,
MicroARNs/metabolismo,
MicroARNs/genética,
Pruebas de Enzimas,
Luciferasas,
Factor 5 de Crecimiento de Fibroblastos/metabolismo,
Recombinación Genética,
Adenoviridae,
Folículo Piloso/citología,
Factor de Necrosis Tumoral alfa/metabolismo,
Folículo Piloso/metabolismo
Background: Using recombinant adeno-associated virus 2 (rAAV-2), we attempted to establish a HEK293T cell line that is able to site-specifically integrate and stably express glial cell line-derived neurotrophic factor (GDNF). Results:Recombinant vector with enhanced green fluorescent protein (EGFP) and G...
O melhoramento genético clássico de sementes milho (Zea mays L.) permitiu desenvolver inúmeras variedades, incluindo o milho com qualidade proteica melhorada (Quality Protein Maize, QPM), que visava aumentar os teores proteicos e as propriedades nutricionais. Por outro lado, novas variedades comerciai...
Background In the present study populations, representing different rounds of recombination were used for the analysis of phenotypic effects associated with the sdw1/denso locus. Other studies have mostly focused only on one type of population. Many different QTLs mapped at the same position as the sdw1/...
Background: Plants differ in the methods used to acquire nutrients from environments with low nutrient availability, and may change the morphology of their root architecture to be able to take up nutrients. Results: In the present study rye response to stress caused by high and low nitrogen-potassium...
Secale/fisiología,
Secale/genética,
Estrés Fisiológico,
Adaptación Fisiológica,
Estrés Oxidativo,
Nitrógeno,
Potasio,
Técnicas In Vitro,
Recombinación Genética,
Fenotipo,
Análisis Multivariante,
Nutrientes
El síndrome Down (SD) es la trisomía más común en humanos, presentándose en 1 de cada 745 nacidos vivos y es la causa más frecuente de retardo mental. El origen más observado de la trisomía es una no disyunción meiótica (95%), la cual generalmente es de origen materno, mientras un 5% se debe a ...
