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Enhanced alkaline catalase production by Serratia marcescens FZSF01: enzyme purification, characterization, and recombinant expression

Jia, Xianbo; Lin, Xinjian; Lin, Chenqiang; Lin, Lirong; Chen, Jichen.
Electron. j. biotechnol; 30 (), 2017
Background: Catalase (CAT) is an important enzyme that degrades H2O2 into H2O and O2. To obtain an efficient catalase, in this study, a new strain of high catalase-producing Serratia marcescens, named FZSF01, was screened and its catalase was purified and characterized. Results: After optimization of fer...
Serratia marcescens/enzimología, Serratia marcescens/genética, Catalasa/metabolismo, Catalasa/aislamiento & purificación, Catalasa/genética, Fermentación, Peróxido de Hidrógeno/metabolismo, ARN Ribosómico 16S, Análisis de Secuencia de ADN, Electroforesis, Cromatografía Liquida, Escherichia coli/genética, Escherichia coli/metabolismo, Recombinación Genética, Cinética
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Draft genome sequence of a GES-5-producing Serratia marcescens isolated in southern Brazil

Nodari, Carolina Silva; Siebert, Marina; Matte, Ursula da Silveira; Barth, Afonso Luís.
Braz. j. microbiol; 48 (2), 2017
Abstract Serratia marcescens is a Gram-negative rod intrinsically resistant to polymyxins and usually associated with wound, respiratory and urinary tract infections. The whole genome of the first GES-5-producing S. marcescens isolated from a Brazilian patient was sequenced using Ion Torrent PGM System. ...
ADN Bacteriano/química, ADN Bacteriano/genética, Genoma Bacteriano, Análisis de Secuencia de ADN, Serratia marcescens/enzimología, Serratia marcescens/genética, beta-Lactamasas/metabolismo, Brasil, Proteínas de Transporte de Membrana/genética, Serratia marcescens/aislamiento & purificación, Transferasas/metabolismo, beta-Lactamasas/genética
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Loop-mediated isothermal amplification assays for screening of bacterial integrons

Yu, Guangchao; Chen, Lei; Lin, Chii-wann; Li, Bing; Cui, Hemiao; Chen, Siyi; Miao, Jian; Bian, Huawei; Chen, Dingqiang; Deng, Yang.
Biol. Res; 47 (), 2014
BACKGROUND: The occurrence and prevalence of integrons in clinical microorganisms and their role played in antimicrobial resistance have been well studied recently. As screening and detection of integrons are concerned, current diagnostic methodologies are restricted by significant drawbacks and novel me...
ADN Bacteriano/aislamiento & purificación, Integrones, Técnicas de Amplificación de Ácido Nucleico/métodos, Farmacorresistencia Bacteriana/genética, Escherichia coli/genética, Integrasas/genética, Reacción en Cadena de la Polimerasa/métodos, Salmonella/genética, Serratia marcescens/genética, Staphylococcus/genética, Vibrio cholerae/genética, Recuento de Colonia Microbiana, Cartilla de ADN, ADN Complementario, Electroforesis en Gel de Agar, Colorantes Fluorescentes, Calor, Pruebas de Sensibilidad Microbiana, Compuestos Orgánicos, Sensibilidad y Especificidad
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