Objectives: Our study aimed to evaluate the stability of human immunodeficiency virus 1 (HIV-1) RNA on cobas plasma separation card (PSC) specimens for viral load (VL) testing after being exposed to varied temperatures and storage times.
Methods: For this purpose, venous PSC specimens were collected and ...
Introduction: Viral load testing is essential to manage HIV disease, especially in infants and children. Early infant diagnosis (EID) is performed using nucleic-acid testing in children under 18 months. Resource-limited health systems face severe challenges to scale-up both viral load and EID to unpreced...
HIV Infections/diagnosis,
HIV Infections/virology,
HIV-1/genetics,
HIV-1/isolation & purification,
HIV-1/physiology,
Infant, Newborn, Diseases/diagnosis,
Infant, Newborn, Diseases/virology,
Mozambique,
Sensitivity and Specificity,
Prospective Studies,
Viral Load/methods,
Viremia/diagnosis,
Viremia/virology
Chissumba, Raquel Matavele;
Luciano, Abílio;
Namalango, Eduardo;
Bauer, Asli;
Bhatt, Nilesh;
Wharen, Britta;
Nilsson, Charlotta;
Geldmacher, Christof;
Scarlatti, Gabriella;
Jani, Ilesh;
Kastens, Luc.
Little is known about regulatory CD4 T cells (Tregs) in the context of HIV vaccines. Tregs can be differentiated into resting (FoxP3+CD45RA+ - rTregs), activated (FoxP3HighCD45RA- - aTregs) and memory (FoxP3LowCD45RA- - mTregs). Tregs, as CD4 T cells, are also frequent targets for HIV infection. We studi...
AIDS Vaccines/immunology,
Antibodies, Neutralizing/immunology,
CD4 Lymphocyte Count,
Cytokines/biosynthesis,
HIV Antibodies/immunology,
HIV Infections/prevention & control,
HIV Infections/virology,
HIV-1/immunology,
HIV-1/genetics,
Mozambique,
CD8-Positive T-Lymphocytes/immunology,
T-Lymphocytes, Regulatory/metabolism,
Vaccines, DNA/immunology,
Vaccines, DNA/genetics
Rupérez, María;
Pou, Christian;
Maculuve, Sonia;
Cedeño, Samandy;
Luis, Leopoldina;
Rodríguez, Judith;
Letang, Emilio;
Moltó, José;
Macete, Eusébio;
Clotet, Boaventura;
Alonso, Pedro;
Menéndez, Clara;
Naniche, Denise;
Paredes, Roger.
Objectives: The objective of this study was to inform public health actions to limit first-line ART failure and HIV drug resistance in Mozambique.
Methods: This was a cross-sectional study. HIV-1-infected adults on first-line ART for at least 1 year attending routine visits in the Manhiça District Hospi...
Anti-Retroviral Agents/therapeutic use,
Drug Monitoring,
Drug Resistance, Viral,
Genotype,
Genotyping Techniques,
HIV Infections/drug therapy,
HIV Infections/virology,
HIV-1/genetics,
HIV-1/isolation & purification,
Suburban Population,
Treatment Failure,
Mozambique,
Drug Resistance
Background: An observational study was conducted in Maputo, Mozambique, to investigate trends in prevalence of HIV drug resistance (HIVDR) in antiretroviral (ART) naïve subjects initiating highly active antiretroviral treatment (HAART).
Methodology/principal findings: To evaluate the pattern of drug res...
Ambulatory Care,
Antiretroviral Therapy, Highly Active,
CD4 Lymphocyte Count,
Drug Resistance, Viral,
HIV Infections/epidemiology,
HIV-1/drug effects,
Host-Pathogen Interactions,
Mutation,
Prevalence,
Treatment Failure,
Viral Load/radiation effects,
Anti-Retroviral Agents/therapeutic use,
Drug Resistance, Viral/genetics,
Genotype,
HIV Infections/rehabilitation,
HIV Infections/virology,
HIV-1/genetics,
HIV-1/physiology,
Viral Load/genetics
Enfuvirtide was the first fusion inhibitor approved by the Food and Drug Administration (FDA) in 2003 for HIV-1 infection in treatment-experienced patient. It is the first approved antiviral agent to attack the HIV life cycle in its early stages. For HIV fusion to occur, the HR1 and HR2 domains in the gp...
HIV-1/genetics,
Amino Acid Sequence,
Anti-HIV Agents,
Cluster Analysis,
Drug Resistance, Viral,
Enfuvirtide,
Genotype,
HIV Envelope Protein gp41/genetics,
HIV Infections/virology,
HIV-1/classification,
Molecular Sequence Data,
Mozambique,
Mutation, Missense,
Peptide Fragments,
Phylogeny,
Polymorphism, Genetic,
RNA, Viral,
Sequence Alignment,
Sequence Analysis, DNA,
Treatment Failure
Bila, Dulce Celina Adolfo;
Young, Pedro;
Merks, Harriet;
Vubil, Adolfo Salvador;
Mahomed, Mussagy;
Augusto, Angelo;
Abreu, Celina Monteiro;
Mabunda, Nédio Jonas;
Brooks, James, I;
Tanuri, Amilcar;
Jani, Ilesh Vinodrai.
Objective: InMozambique, highly active antiretroviral treatment (HAART) was introduced in 2004 followed by decentralizationandexpansion, resulting inamorethan20-fold increase incoverageby2009. ImplementationofHIV drugresistancethresholdsurveys (HIVDR-TS) iscrucial inorder tomonitor theemergenceof transmi...
Anti-HIV Agents/pharmacology,
Anti-HIV Agents/therapeutic use,
Antiretroviral Therapy, Highly Active,
Mozambique,
Drug Resistance, Viral/genetics,
HIV Infections/therapy,
HIV Infections/epidemiology,
HIV Infections/virology,
HIV-1/classification,
HIV-1/radiation effects,
HIV-1/genetics,
Mozambique/epidemiology,
Mutation,
Phylogeny
Jani, Ilesh;
Sebastian, Jennifer;
Vubil, Adolfo;
Subbarao, Shambavi;
Bila, Dolce;
Sousa, Amina;
Mabunda, Nédio;
Garcia, Albert;
Ellemberger, Dennis;
Ramos, Artur;
Skaggs, Beth.
We performed a comparative analysis between Roche Amplicor HIV-1 DNA test and CAPTAQ assay for the detection of HIV in 830 dried blood spot (DBS) pediatric samples collected in Mozambique. Our results demonstrated no statistical difference between these assays. The CAPTAQ assay approached nearly 100% rep...
Vaz, paula;
Chaix, marie-laure;
Jani, ilesh;
Macassa, eugenia;
Bila, Dulce;
Vubil, Adolfo;
Anderson, Soren;
Rouzioux, christine, pharmD;
Briand, nelly;
Blanche, stephane.
Background: Resistant virus may be selected by sub-optimal control of HIV-1 replication during antiretroviral treatment. The incidence and profile of resistance in children receiving World Health Organization-recommended treatment remains to be evaluated on a large scale.
Goals: Assessment of the frequen...
Drug Resistance,
HIV-1/genetics,
Anti-HIV Agents,
HIV Infections/blood,
Virus Replication,
Analysis of Variance,
HIV-1/physiology,
HIV Infections/therapy,
HIV Infections/virology,
Reverse Transcriptase Inhibitors/pharmacology,
Reverse Transcriptase Inhibitors/therapeutic use,
Anti-HIV Agents/pharmacology,
Anti-HIV Agents/therapeutic use,
Treatment Failure,
Mozambique
Ismael, Nalia;
Wilkinson, Eduan;
Mahumane, Isabel;
Mahumane, Isabel;
Gemusse, Hernanane;
Giandhari, Jennifer;
Bauhofer, Adilson;
Vubil, Adolfo;
Mambo, Pirolita;
Singh, Lavanya;
Mabunda, Nédio;
Bila, Dulce;
Engelbrecht, Susan;
Gudo, Eduardo;
Lessells, Richard;
Oliveira, Túlio.
HIV drug resistance (HIVDR) can become a public health concern, especially in low- and middle-income countries where genotypic testing for people initiating antiretroviral therapy (ART) is not available. For first-line regimens to remain effective, levels of transmitted drug resistance (TDR) need to be m...
Mozambique/epidemiology,
HIV-1/genetics,
Drug Resistance, Viral/genetics,
Anti-HIV Agents/therapeutic use,
Anti-HIV Agents/pharmacology,
HIV Infections/drug therapy,
HIV Infections/epidemiology,
HIV Seropositivity,
Molecular Epidemiology,
Reverse Transcriptase Inhibitors/pharmacology,
Prevalence
