Koofhethile, Catherine;
Rinaldi, Stefano;
Rassadkina, Yelizaveta;
Dinh, Vinh B;
Gao, Ce;
Pallikkuth, Suresh;
Broncano, Pilar Garcia;
Armas, Lesley R de;
Pahwa, Rajendra;
Cotugno, Nicola;
Vaz, Paula;
Lain, Maria Grazia;
Palma, Paolo;
G Yu, Xu;
Shapiro, Roger;
Pahwa, Savita;
Lichterfeld, Mathias.
With more than 1.7 million infected children worldwide, pediatric HIV-1 infection remains
a global health challenge; in 2020 alone, current estimates suggest that more than 150,000
children were newly infected with HIV-1 [1]. Most of these children are infected through
breastfeeding, but vertical mother-...
Objectives: Our study aimed to evaluate the stability of human immunodeficiency virus 1 (HIV-1) RNA on cobas plasma separation card (PSC) specimens for viral load (VL) testing after being exposed to varied temperatures and storage times.
Methods: For this purpose, venous PSC specimens were collected and ...
Introduction: Viral load testing is essential to manage HIV disease, especially in infants and children. Early infant diagnosis (EID) is performed using nucleic-acid testing in children under 18 months. Resource-limited health systems face severe challenges to scale-up both viral load and EID to unpreced...
HIV Infections/diagnosis,
HIV Infections/virology,
HIV-1/genetics,
HIV-1/isolation & purification,
HIV-1/physiology,
Infant, Newborn, Diseases/diagnosis,
Infant, Newborn, Diseases/virology,
Mozambique,
Sensitivity and Specificity,
Prospective Studies,
Viral Load/methods,
Viremia/diagnosis,
Viremia/virology
Chissumba, Raquel Matavele;
Luciano, Abílio;
Namalango, Eduardo;
Bauer, Asli;
Bhatt, Nilesh;
Wharen, Britta;
Nilsson, Charlotta;
Geldmacher, Christof;
Scarlatti, Gabriella;
Jani, Ilesh;
Kastens, Luc.
Little is known about regulatory CD4 T cells (Tregs) in the context of HIV vaccines. Tregs can be differentiated into resting (FoxP3+CD45RA+ - rTregs), activated (FoxP3HighCD45RA- - aTregs) and memory (FoxP3LowCD45RA- - mTregs). Tregs, as CD4 T cells, are also frequent targets for HIV infection. We studi...
AIDS Vaccines/immunology,
Antibodies, Neutralizing/immunology,
CD4 Lymphocyte Count,
Cytokines/biosynthesis,
HIV Antibodies/immunology,
HIV Infections/prevention & control,
HIV Infections/virology,
HIV-1/immunology,
HIV-1/genetics,
Mozambique,
CD8-Positive T-Lymphocytes/immunology,
T-Lymphocytes, Regulatory/metabolism,
Vaccines, DNA/immunology,
Vaccines, DNA/genetics
Megg, Bindiya;
Vojnov, Lara;
Mabunda, Nédio;
Vubil, Adolfo;
Zitha, Alcina;
Tobaiwa, Ocean;
Mudenyanga, Chishamiso;
Mutsaka, Dadirayi;
Bollinger, Timothy;
Loquiha, Osvaldo;
Pete, Trevor F;
Jan, Ilesh V.
Worldwide only 43% of infants infected with HIV have access to antiretroviral treatment (ART). Despite significant global efforts to expand access to paediatric treatment, coverage lags behind adult ART and may not reach the 90-90-90 goals by 2020 as set by UNAIDS [2]. Resolving this paediatric ART gap i...
Rupérez, María;
Pou, Christian;
Maculuve, Sonia;
Cedeño, Samandy;
Luis, Leopoldina;
Rodríguez, Judith;
Letang, Emilio;
Moltó, José;
Macete, Eusébio;
Clotet, Boaventura;
Alonso, Pedro;
Menéndez, Clara;
Naniche, Denise;
Paredes, Roger.
Objectives: The objective of this study was to inform public health actions to limit first-line ART failure and HIV drug resistance in Mozambique.
Methods: This was a cross-sectional study. HIV-1-infected adults on first-line ART for at least 1 year attending routine visits in the Manhiça District Hospi...
Anti-Retroviral Agents/therapeutic use,
Drug Monitoring,
Drug Resistance, Viral,
Genotype,
Genotyping Techniques,
HIV Infections/drug therapy,
HIV Infections/virology,
HIV-1/genetics,
HIV-1/isolation & purification,
Suburban Population,
Treatment Failure,
Mozambique,
Drug Resistance
Jani, Ilesh;
Sebastian, Jennifer;
Vubil, Adolfo;
Subbarao, Shambavi;
Bila, Dolce;
Sousa, Amina;
Mabunda, Nédio;
Garcia, Albert;
Ellemberger, Dennis;
Ramos, Artur;
Skaggs, Beth.
We performed a comparative analysis between Roche Amplicor HIV-1 DNA test and CAPTAQ assay for the detection of HIV in 830 dried blood spot (DBS) pediatric samples collected in Mozambique. Our results demonstrated no statistical difference between these assays. The CAPTAQ assay approached nearly 100% rep...
Vaz, paula;
Chaix, marie-laure;
Jani, ilesh;
Macassa, eugenia;
Bila, Dulce;
Vubil, Adolfo;
Anderson, Soren;
Rouzioux, christine, pharmD;
Briand, nelly;
Blanche, stephane.
Background: Resistant virus may be selected by sub-optimal control of HIV-1 replication during antiretroviral treatment. The incidence and profile of resistance in children receiving World Health Organization-recommended treatment remains to be evaluated on a large scale.
Goals: Assessment of the frequen...
Drug Resistance,
HIV-1/genetics,
Anti-HIV Agents,
HIV Infections/blood,
Virus Replication,
Analysis of Variance,
HIV-1/physiology,
HIV Infections/therapy,
HIV Infections/virology,
Reverse Transcriptase Inhibitors/pharmacology,
Reverse Transcriptase Inhibitors/therapeutic use,
Anti-HIV Agents/pharmacology,
Anti-HIV Agents/therapeutic use,
Treatment Failure,
Mozambique
Ismael, Nalia;
Wilkinson, Eduan;
Mahumane, Isabel;
Mahumane, Isabel;
Gemusse, Hernanane;
Giandhari, Jennifer;
Bauhofer, Adilson;
Vubil, Adolfo;
Mambo, Pirolita;
Singh, Lavanya;
Mabunda, Nédio;
Bila, Dulce;
Engelbrecht, Susan;
Gudo, Eduardo;
Lessells, Richard;
Oliveira, Túlio.
HIV drug resistance (HIVDR) can become a public health concern, especially in low- and middle-income countries where genotypic testing for people initiating antiretroviral therapy (ART) is not available. For first-line regimens to remain effective, levels of transmitted drug resistance (TDR) need to be m...
Mozambique/epidemiology,
HIV-1/genetics,
Drug Resistance, Viral/genetics,
Anti-HIV Agents/therapeutic use,
Anti-HIV Agents/pharmacology,
HIV Infections/drug therapy,
HIV Infections/epidemiology,
HIV Seropositivity,
Molecular Epidemiology,
Reverse Transcriptase Inhibitors/pharmacology,
Prevalence
